Amini-Sarteshnizi, Nematallah. and Teimori, Hossein. and Beshkar, Pezhman. and Amini-Sarteshnizi, Roghayeh. and Nikoukar, Morteza. (2016) Study of CAPE effect on apoptosis induction in AGS human gastric cancer cell line. Jundishapur Journal of Natural Pharmaceutical Products, 11 (1).
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Abstract
Background: Propolis is a natural product of bee and caffeic acid phenethyl ester (CAPE) is a pharmacologically important product of propolis. Objectives: The aim of this study was to investigate the effect of CAPE on apoptosis induction in AGS human gastric cancer cells. Materials and Methods: The cytotoxic effects of CAPE at different concentrations were investigated on AGS cells viability after 24 hours treatment by MTT assay. To measure the effect of CAPE on apoptosis induction, AGS cells were treated with CAPE for 24 hours and investigated by FITC Annexin V/PI staining using flow cytometry. Results: CAPE prevented growth and proliferation of AGS human gastric cancer cell line in a concentration-dependent manner with an IC50 of approximately 60 μM by a 24-hour treatment. Also CAPE caused increased induction of apoptosis in AGS cells from 1.37 % in control cells to 21.76 % in treated cells with 30 μM CAPE. Conclusions: CAPE prevents growth and proliferation of AGS human gastric cancer cell line through inducing programmed cell death in AGS cells. Therefore, CAPE could be helpful for developing chemotherapeutic agents or as an adjuvant for human gastric cancer treatment. © 2016, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences.
Item Type: | Article |
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Additional Information: | cited By 0 |
Uncontrolled Keywords: | Apoptosis, Caffeic acid phenethyl ester, Cancer, Cell survival |
Subjects: | WI Digestive System QZ pathology-Neoplasms QV pharmacology > QV 752 Pharmacognosy |
Divisions: | Reserach Vice-Chancellar Department > Cellular and Molecular Research Center Reserach Vice-Chancellar Department > Medical Plants Research Center |
Depositing User: | Users 1 not found. |
Date Deposited: | 15 Jul 2017 04:06 |
Last Modified: | 07 Feb 2018 10:17 |
URI: | http://eprints.skums.ac.ir/id/eprint/987 |
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