Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva

Taghipour, Simin and Abastabar, Mahdi and Piri, Fahimeh and Aboualigalehdari, Elham and Jabbari, Mohammad Reza and Zarrinfar, Hossein and Nouripour-Sisakht, Sadegh and Mohammadi, Rasoul and Ahmadi, Bahram and Ansari, Saham and Katiraee, Farzad and Niknejad, Farhad and Didehdar, Mojtaba and Nazeri, Mehdi and Makimura, Koichi and Rezaei-Matehkolaei, Ali (2021) Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva. JOURNAL OF FUNGI, 7 (5). ISSN 2309-608X

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Abstract

A molecular epidemiology study was conducted between 2016 and 2017 by a network of collaborators from 12 provinces in the Islamic Republic of Iran. A total of 1484 soil samples from different habitats were screened for the presence of dermatophytes by using the hair baiting technique. The primary identification of isolates was carried out by amplification and MvaI restriction fragment length polymorphism (RFLP) of the internal transcribed spacers regions of ribosomal DNA (ITS-rDNA). The identifications, especially in the cases of isolates with unknown RFLP patterns, were confirmed by sequencing of the ITS-rDNA region. As a result, 256 isolates were recovered. The isolation rate was higher in soils with pH range 7.1-8.0, collected from animal habitats (n = 78; 34%) and parks and gardens (n = 75; 32%), geographically from Mazandaran Province (n = 115; 49.5%) and seasonally in the spring (n = 129; 50.4%), all of which were statistically significant (p < 0.05). The dermatophytes comprising five species of the two genera, viz., Nannizzia fulva (n = 214), N. gypsea (n = 34), Arthroderma quadrifidum (n = 5), A. gertleri (n = 2) and A. tuberculatum (n = 1), were isolated. The geophilic dermatophytes occurred in various soils from different parts of Iran; however, surprisingly, N. fulva emerged as the dominant species, outnumbering the common geophilic species of N. gypsea. For the definitive identification of soil inhabitant dermatophytes, DNA-based identification is strongly recommended.

Item Type: Article
Uncontrolled Keywords: geophilic dermatophytes; Nannizzia fulva; Arthroderma; ITS sequencing; Iran
Subjects: QX Parasitology
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Parasitology and Mycology
Depositing User: zeynab . bagheri
Date Deposited: 30 May 2021 04:17
Last Modified: 30 May 2021 04:17
URI: http://eprints.skums.ac.ir/id/eprint/9015

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