Defining the role of 17 beta-estradiol in human endometrial stem cells differentiation into neuron-like cells

Hasanzadeh, Elham and Ebrahimi-Barough, Somayeh and Mahmoodi, Narges and Mellati, Amir and Nekounam, Houra and Basiri, Arefeh and Asadpour, Shiva and Ghasemi, Diba and Ai, Jafar (2020) Defining the role of 17 beta-estradiol in human endometrial stem cells differentiation into neuron-like cells. CELL BIOLOGY INTERNATIONAL, 45 (1). pp. 140-153.

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Human endometrial stem cells (hEnSCs) that can be differentiated into various neural cell types have been regarded as a suitable cell population for neural tissue engineering and regenerative medicine. Considering different interactions between hormones, growth factors, and other factors in the neural system, several differentiation protocols have been proposed to direct hEnSCs towards specific neural cells. The 17 beta-estradiol plays important roles in the processes of development, maturation, and function of nervous system. In the present research, the impact of 17 beta-estradiol (estrogen, E2) on the neural differentiation of hEnSCs was examined for the first time, based on the expression levels of neural genes and proteins. In this regard, hEnSCs were differentiated into neuron-like cells after exposure to retinoic acid (RA), epidermal growth factor (EGF), and also fibroblast growth factor-2 (FGF2) in the absence or presence of 17 beta-estradiol. The majority of cells showed a multipolar morphology. In all groups, the expression levels of nestin, Tuj-1 and NF-H (neurofilament heavy polypeptide) (as neural-specific markers) increased during 14 days. According to the outcomes of immunofluorescence (IF) and real-time PCR analyses, the neuron-specific markers were more expressed in the estrogen-treated groups, in comparison with the estrogen-free ones. These findings suggest that 17 beta-estradiol along with other growth factors can stimulate and upregulate the expression of neural markers during the neuronal differentiation of hEnSCs. Moreover, our findings confirm that hEnSCs can be an appropriate cell source for cell therapy of neurodegenerative diseases and neural tissue engineering.

Item Type: Article
Subjects: QU Biochemistry
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Advanced Technologies > Department of Tissue Engineering
Depositing User: zeynab . bagheri
Date Deposited: 15 Feb 2021 08:49
Last Modified: 15 Feb 2021 08:49

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