Diosgenin via NMDA Receptor Exerted Anxiolytic-like Effect in Maternally Separated Mice

Boroujeni, Shakiba Nasiri and Lorigooini, Zahra and Boldaji, Fatemeh Rahimi and Amini-Khoei, Hossein (2020) Diosgenin via NMDA Receptor Exerted Anxiolytic-like Effect in Maternally Separated Mice. Current Pharmaceutical Design, 26. ISSN 13816128

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Official URL: https://pubmed.ncbi.nlm.nih.gov/32679011/

Abstract

Background and aim: Anxiety is one of the most common psychiatric disorders that lead to disruption of daily and quality of life. Routine medications have many side effects and cause physical dependence and psychosocial addiction. Diosgenin is a phytosteroid found in a number of herbs. The aim of present study was to investigate the anxiolytic-like effect of diosgenin in the maternal separation model in male mice focusing on the role of NMDA receptors. Material and methods: Maternal separation (MS) paradigm was performed daily (3 h) from postnatal day (PND) 2-14. Male mice were treated with different dose of diosgenin to find effective and sub-effective doses. In the next step, mice were treated with effective dose of diosgenin plus NMDA and or sub-effective dose of diosgenin plus ketamine (NMDA antagonist). Valid behavioral test for evaluation of anxiety-like behavior were performed. Then, mice were killed, hippocampus dissected out and gene expression of NMDA receptors (NR2a and NR2b subunits) was assessed. Results: MS provokes anxiety-like behaviors in the open field test (OFT) and elevated plus maze (EPM). Diosgenin significantly mitigated negative effects of MS. Co-administration of NMDA attenuated anxiolytic-like effect of effective dose of diosgenin while ketamine potentiated the anxiolytic effect of sub-effective dose of diosgenin. Furthermore, MS increased the expression of NMDA receptor in the hippocampus which to some extent modulated with diosgenin. Conclusion: Diosignin has anxiolytic-like effect in MS mice which at least, in part, mediated through NMDA receptors. Keywords: Anxiety; Diosignin; Maternal separation; NMDA receptor; mice.

Item Type: Article
Subjects: WL Nervous system
QV pharmacology > QV 767 Traditional Pharmaceutics
Divisions: Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zeynab . bagheri
Date Deposited: 20 Jul 2020 07:11
Last Modified: 20 Jul 2020 07:11
URI: http://eprints.skums.ac.ir/id/eprint/8625

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