Resveratrol-loaded polyurethane nanofibrous scaffold: Viability of endothelial and smooth muscle cells

Asadpour, Shiva and Yeganeh, Hamid and Khademi, Farzaneh and Ghanbari, Hossein and Ai, Jafar (2020) Resveratrol-loaded polyurethane nanofibrous scaffold: Viability of endothelial and smooth muscle cells. Biomedical Materials (Bristol), 15 (1). ISSN 17486041

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Abstract

Acellular small-caliber tissue-engineered vascular grafts (SCTEVGs) have low patency rate due to complications including thrombosis and intimal hyperplasia. Rapid endothelialization, antithrombosis and antiproliferation approaches are suitable for dispelling these complications. Nevertheless, common antithrombosis and antiproliferation techniques are usually incompatible with rapid endothelialization on vascular grafts. To overcome these obstacles, we developed nanofibrous polyurethane scaffolds loaded with resveratrol drug, which is a natural compound extracted from plants and shows multifaceted effects in cardiovascular protection. It was found that the tensile strength and Young's modulus in modified scaffolds were significantly increased by resveratrol loading into membranes. The tensile strengths and breaking strains of resveratrol-loaded scaffolds were close to that of native vessels. The resveratrol release profile from the nanofibrous scaffolds occurred in a sustained manner. The anti-thrombogenicity of resveratrol-loaded nanofibers increased compared to polyurethane alone, with the result that prolonged human blood clotting time and lower hemolysis were detected on these scaffolds. The viability of human umbilical vein endothelial cells and smooth muscle cells on resveratrol-loaded scaffolds was evaluated. Our findings demonstrated that resveratrol-loaded nanofibers resulted in not only appropriate antithrombotic properties, but the formation of a monolayer of endothelial cells on the scaffold surface and lower smooth muscle cell growth. These resveratrol-loaded nanofibers are suggested as potential scaffolds for SCTEVGs.

Item Type: Article
Uncontrolled Keywords: Blood; Cell proliferation; Cytology; Drug delivery; Elastic moduli; Endothelial cells; Grafts; Muscle; Nanofibers; Polyurethanes; Resveratrol; Tensile strength, Anti-thrombogenicity; Blood compatibility; Cardiovascular protections; Endothelialization; Human umbilical vein endothelial cells; Nanofibrous scaffolds; Polyurethane scaffolds; Tissue engineered vascular grafts, Scaffolds (biology), molecular scaffold; polyurethan; resveratrol, Article; breaking strength; cell viability; endothelium cell; human; human cell; HUVEC cell line; smooth muscle cell; tensile strength
Subjects: QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Advanced Technologies > Department of Tissue Engineering
Depositing User: zeynab . bagheri
Date Deposited: 26 May 2020 06:25
Last Modified: 02 Jun 2020 06:01
URI: http://eprints.skums.ac.ir/id/eprint/8508

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