Serum 25-hydroxyvitamin D level and vitamin D receptor (VDR) polymorphisms in patients infected with Leishmania tropica: a case control study

Shabandoust, Hajar and Sharifi, Iraj and Raiesi, Omid and Getso, Muhammad Ibrahim and Dezaki Saedi, Ebrahim and Afgar, Ali and Shirvani, Giti and Salarkia, Ehsan and Babaei, Zahra (2020) Serum 25-hydroxyvitamin D level and vitamin D receptor (VDR) polymorphisms in patients infected with Leishmania tropica: a case control study. Journal of Parasitic Diseases, 44 (1). pp. 40-48. ISSN 09717196

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Abstract

Leishmania, an obligate intracellular parasite is eliminated by a strong Th-1 host response. As Vitamin D metabolism and its receptor activity are important factors in human native immune system against some microorganisms, we hypothesized that VDR gene polymorphisms and concentration of Vitamin D might have effect on incidence of cutaneous leishmaniasis. The aim of this study was to investigate the association between VDR gene polymorphism and/or the serum vitamin D level and leishmaniasis in the infected patients in comparison to the healthy individuals. In this case–control study, the BsmI, FokI and Taq1 polymorphisms in the VDR gene and serum levels of vitamin D were studied in Iranian infected with Leishmania tropica (n = 50) and healthy controls using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) and Electrochemiluminescence methods respectively. Data were statistically analyzed using SPSS software, Chi square and ANOVA tests. The results of this study showed that despite the relatively higher frequency of BsmI-BB, FokI-FF and TaqI-Tt than Non BsmI-BB, Non FokI-FF and Non TaqI-Tt in the patients compared with the healthy individuals, the differences were not statistically significant (P > 0.05). Based on our findings, the relationship between the VDR polymorphism, the serum concentration of 25-hydroxyvitamin D and the susceptibility to Leishmania tropica infection, remains unclear requiring further in-depth studies. However, for better interpretation, it is necessary to consider factors such as the size of the sample examined and the other alleles of VDR, including Apal.

Item Type: Article
Uncontrolled Keywords: 25 hydroxyvitamin D; vitamin D receptor, adolescent; Article; blood sampling; case control study; child; clinical article; controlled study; DNA extraction; DNA polymorphism; electrochemiluminescence; electrophoresis; female; gene; human; human cell; human tissue; Leishmania tropica; leishmaniasis; leukocyte; male; microscopy; normal human; polymerase chain reaction restriction fragment length polymorphism; priority journal; questionnaire; skin leishmaniasis; VDR
Subjects: WR Dermatology
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Parasitology and Mycology
Depositing User: zeynab . bagheri
Date Deposited: 27 May 2020 09:37
Last Modified: 27 May 2020 09:37
URI: http://eprints.skums.ac.ir/id/eprint/8465

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