Genetically modified mice-methods, applications and outlook

Eskandarian Boroujeni, Mahdi and Alizadeh Miran, Rafieh and Afkhami, Fatemeh and Tavakol, Zeinab and Shamsara, Mehdi (2019) Genetically modified mice-methods, applications and outlook. Scientific Journal of Kurdistan University of Medical Sciences, 24 (1). ISSN 1560652X

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Official URL: https://www.scopus.com/record/display.uri?eid=2-s2...

Abstract

Background & Aim: Transgenic mice, of tengenerated by random integration of foreign genes into the mouse genome or by targeted mutation in a particular gene, have demonstrated to be a very effective tool for studying gene function in living things. In this review article, we discussed on the current methods of generating genetically-modified mice and their related problems and then investigated the new methods developed to overcome these problems. Finally, we discussed future prospects on the gene targeting. Methods & Materials: This is a review article, which has been written after searching Pubmed, Scopus, Google Scholar, Springer, Elsevier and Magiran databases by using keywords of transgenic mice, functional genetics, genetargeting, and homologous recombination. Results: This study dealt with genetic variations in a wide range, differential processing and inactivation of gene-specific isoforms, local and induced genetic changes, Cre/loxP system and some future perspectives. Conclusion: Success rate in genetic modification of mouse genome has increased dramatically, and use of knockout mice has resulted in increased knowledge of human biology and diseases.

Item Type: Article
Subjects: QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Nursing and Midwifery > Department of Midwifery
Depositing User: zeynab . bagheri
Date Deposited: 18 May 2020 06:51
Last Modified: 18 May 2020 06:51
URI: http://eprints.skums.ac.ir/id/eprint/8392

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