Natural biomacromolecule based composite scaffolds from silk fibroin, gelatin and chitosan toward tissue engineering applications

Asadpour, Shiva and Kargozar, Saeid and Moradi, Lida and Ai, Armin and Nosrati, Hamed and Ai, Jafar (2019) Natural biomacromolecule based composite scaffolds from silk fibroin, gelatin and chitosan toward tissue engineering applications. International Journal of Biological Macromolecules, 154. pp. 1285-1294. ISSN 01418130

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Abstract

Natupolymer-based scaffolds can increase cell affinity to biomaterials and improve cell responses. Silk fibroin, chitosan and gelatin that mimic the properties of natural extra-cellular matrix (ECM) were chosen due to their biocompatibility, biodegradability and less immunogenic reactions. We prepared composite scaffolds with different blending ratios of silk fibroin-chitosan-gelatin by freeze-drying technique. Silk fibroin was extracted from the Bombyx mori silkworm. The scaffolds were characterized by scanning electron microscopy (SEM), surface wettability, swelling measurements, In Vitro enzymatic degradation measurements and tensile test. The composite scaffolds showed pore sizes from 125 μm to 175 μm, good interconnectivity between pores and suitable porosity which are desirable for cell growth. The addition of chitosan-gelatin to silk fibroin increased water uptake and degradation rate and reduced mechanical strength but silk fibroin affect reversely on the degradation and mechanical strength of composite scaffolds. Biocompatibility of scaffolds was demonstrated by MTT-assay and hematoxylin-eosin (H&E) staining which lead to the growth and adhesion of endothelial cells. In this study, the fabricated composite scaffolds have the potential for tissue engineering applications.

Item Type: Article
Subjects: QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Advanced Technologies > Department of Tissue Engineering
Depositing User: zeynab . bagheri
Date Deposited: 18 May 2020 05:08
Last Modified: 02 Jun 2020 06:04
URI: http://eprints.skums.ac.ir/id/eprint/8383

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