Altered Th17 Cytokine Expression in Helicobacter pylori Patients with TLR4 (D299G) Polymorphism

Bagheri, N. and Azadegan-Dehkordi, F. and Rahimian, G. and Hashemzadeh-Chaleshtori, M. and Rafieian-Kopaei, M. and Kheiri, S. and Gholipour, A. and Shirzad, H. (2016) Altered Th17 Cytokine Expression in Helicobacter pylori Patients with TLR4 (D299G) Polymorphism. Immunological Investigations, 45 (2). pp. 161-171.

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Abstract

Helicobacter pylori (H. pylori) is associated with gastric ulcer and gastric adenocarcinoma. Polymorphisms in the host genes coding for Toll-like receptors (TLRs) may influence the innate and adaptive immune response to the infection, affecting the susceptibility to H. pylori or the disease outcomes. However, the details and association with different polymorphism and clinical expression of infection remain unclear. A case-control study consisting of 58 patients with H. pylori infection and 44 H. pylori uninfection was conducted. Genomic DNA was extracted and genotypes of TLR4 Asp299Gly polymorphism were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Mucosal cytokines expression in H. pylori-infected and uninfected gastric biopsies was determined by real-time PCR. The expression of IL-6, IL-17, IL-21, IL-23 and TGF-β1 was signicantly higher in patients with D299G polymorphism in TLR4. But the expression of IL-18 between patients with single-nucleotide polymorphisms (SNPs) in TLR4 and patients with the wild-type allele was not signicant. In H. pylori-infected patients with gastritis, SNPs in TLR4 may alter cytokine expression toward Th17 immune response in the gastric mucosa and may have increased risk for the development of peptic ulcer.

Item Type: Article
Additional Information: cited By 8
Uncontrolled Keywords: Cytokine, gastritis, gene polymorphism, Helicobacter pylori, Th17, TLR4
Subjects: WI Digestive System
QU Biochemistry
Divisions: Faculty of Health > Department of Epidemiology
Faculty of Medicine > Department of Clinical Sciences > Department of Internal Medicine
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 08 Jul 2017 04:37
Last Modified: 08 Jul 2017 04:37
URI: http://eprints.skums.ac.ir/id/eprint/823

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