Assessment of the toxicity effects of nicotine on sperm and IVF and the potential protective role of silymarin—an experimental study in mice

Rahimi-Madiseh, Mohammad and Mohammadi, Mohsen and Hassanvand, Amin and Ahmadi, Reza and Shahmohammadi, Maasume and Rostamzadeh, Ayoob (2020) Assessment of the toxicity effects of nicotine on sperm and IVF and the potential protective role of silymarin—an experimental study in mice. Middle East Fertility Society Journal, 25 (1).

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Abstract

Abstract Background: Male infertility is usually caused via the inability to produce adequate quantities of healthy and active sperms. Nicotine (NIC) is an alkaloid organic compound, predominantly found in the leaves of the tobacco plant. The major part of the nicotine is not ionized, so it can easily pass through cell membranes. Meanwhile, most of the disorders are caused by oxidative stress due to oxygen free radicals and other reactive species. Antioxidant supplements and antioxidant-rich foods can reduce induced oxidative stress without becoming destabilized themselves. This study aimed to examine spermato-protective potential of silymarin (SIL), on sperm and in vitro fertility (IVF) rate in nicotine-treated mice. Results: Our results show a significant increase in the number of abnormal sperm morphology after nicotine exposure, when compared to control groups (p < 0.05). On the other hand, SIL had a significant effect on the sperm count at each of the treated doses. Further, in the mice that received nicotine plus silymarin, the viable sperm percentage and the progressive sperm motility were significant (p < 0.05). Also, a significant reduction in the number of two-cell embryos and blastocyst-derived embryo was seen with increment in the number of dead embryos in mice receiving nicotine alone (p < 0.05). Conclusions: In conclusion, SIL could support prevention of the adverse reproductive effects of nicotine. Moreover, SIL200 mg/kg may be therefore considered as a spermato-protective agent in dietary and herbal supplements. © 2020, The Author(s). Author:Antioxidant, Fertility, In vitro fertility, Nicotine, Silymarin, Spermatogenesis

Item Type: Article
Subjects: WJ Urogenital System
Divisions: Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zeynab . bagheri
Date Deposited: 20 Apr 2020 05:01
Last Modified: 20 Apr 2020 05:01
URI: http://eprints.skums.ac.ir/id/eprint/8171

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