Genetically modified Pichia pastoris, a powerful resistant factory for gold and palladium bioleaching and nanostructure heavy metal biosynthesis

Elahian, Fatemeh and Heidari, Razieh and Charghan, Vahid Reza and Asadbeik, Elham and Mirzaei, Seyed Abbas (2020) Genetically modified Pichia pastoris, a powerful resistant factory for gold and palladium bioleaching and nanostructure heavy metal biosynthesis. Artificial Cells, Nanomedicine and Biotechnology, 48 (1). ISSN 21691401

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Abstract

A metal-resistant engineered Pichia pastoris was developed here to fulfil the metal bioleaching in aqueous conditions. Parent and recombinant yeasts were grown in YPD medium containing different concentrations of ion metals. XRD, electron microscopy and particle size analyser were used for the characterisation and the nanoparticle analyses. The nanoparticle production kinetics were studied by ICP-OES. The cytotoxicity of nanoparticles was assayed against human cell lines. Media colours changed to a range from purplish-brown to grey during early fermentation stages. The maximum biosorption capacities were recorded 81.23 and 493.35 mg/g for gold and palladium in batch conditions, respectively. Various physical investigations proved monodispersed spherical nanoparticles around 100 nm in size. Pure palladium nanoparticles and PdCl2 represented the least cytotoxic potency towards T47D and EPG85.257 cells. The results demonstrated that the genetically modified yeast is a cost-effective, high-throughput, robust, and facile system for metal biosorption. KEYWORDS: Biosorption; bioleaching; gold; palladium nanoparticle; recombinant Pichia pastoris

Item Type: Article
Subjects: QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Advanced Technologies > Department of Medical Biotechnology
Depositing User: zeynab . bagheri
Date Deposited: 05 Feb 2020 08:21
Last Modified: 02 Jun 2020 05:57
URI: http://eprints.skums.ac.ir/id/eprint/8107

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