Increased levels of miR-124 in human dental pulp stem cells alter the expression of neural markers

Mehri-Ghahfarrokhi, Ameneh and Pourteymourfard-Tabrizi, Zahra and Farrokhi, Effat and Hashemzadeh Chaleshtori, Morteza and Jami, Mohammad-Saeid (2019) Increased levels of miR-124 in human dental pulp stem cells alter the expression of neural markers. Journal of Otology.

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Abstract

Auditory neuropathy is the particular form of deafness in humans which cannot be treated by replacement therapy. Human dental pulp stem cells (hDPSCs) are derived from an ectomesenchymal neural crest cell population. Therefore, they possess a promising capacity for neuronal differentiation and repair. miR-124, a key regulator of neuronal development in the inner ear, is expressed at high levels in auditory and vestibular neurons. Here, we evaluated the possible effect of miR-124 in alteration of neural protein markers expression. Using quantitative reverse transcription-PCR (qRT-PCR) analyses and immunofluorescence staining, we studied the expression patterns of neural progenitor markers (Nestin, NOTCH1, and SOX2) and neural markers (β-tubulin III, GATA-3, and peripherin) upon transfection of hDPSCs with miR-124. The qRT-PCR results showed that Nestin was upregulated 6 h post-transfection. In contrast, Nestin expression exhibited a decreasing trend 24 h and 48 h post-transfection. Higher levels of β-tubulin III, 6 h and 16 h post transfection in RNA level as compared with control cells, were determined in transfected DPSCs. However, β-tubulin-III expression decreased 48 h post-transfection. The immunoflourescence results indicated that transfection of hDPSCs with miR-124, only affected Nestin among the studied neural progenitor and neural marker expression in protein level.

Item Type: Article
Subjects: WU Dentistry. Oral surgery
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Advanced Technologies > Department of Molecular Medicine
Depositing User: marzieye nazari .
Date Deposited: 11 Dec 2019 06:20
Last Modified: 19 May 2020 05:44
URI: http://eprints.skums.ac.ir/id/eprint/8048

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