The impact of caffeic acid phenethyl ester, chrysin and ethanolic extracts of propolis on PLD1 gene expression in AGS cell line

Jafari-Ghahfarokhi, Hamideh and Jazaeri, Ali and Amini-Sarteshnizi, Nematallah and Teimori, Hossein (2019) The impact of caffeic acid phenethyl ester, chrysin and ethanolic extracts of propolis on PLD1 gene expression in AGS cell line. Journal of Herbmed Pharmacology.

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Abstract

Introduction: Up-regulation of phospholipase D (PLD) is functionally related to tumorigenesis and oncogenic signals. Chrysin, caffeic acid phenethyl ester (CAPE) and ethanolic extract of propolis (EEP) are three safe compounds which have been shown to possess antiproliferative, antioxidant, anti-inflammatory and antineoplastic properties. In this study, the effects of these three compounds on PLD1 gene expression were examined in AGS cell line. Methods: After determining the appropriate concentrations of EEP, CAPE, and chrysin, AGS cells were cultured in mediums with proper ratios of the compounds. AGS cells were maintained in exponential growth and the culture mediums refreshed every other day. Finally, after extracting total RNA from AGS cells, real-time PCR was performed to evaluate the mRNA expression of PLD1 in the presence of each compound. Results: CAPE decreased the mRNA level of PLD1 gene in a dose-dependent manner. A solution with 30 μM concentration of CAPE was the effective dose in comparison to control group as well as 15 and 20 μM concentrations of the compound, whereas no changes were observed in the presence of EEP and chrysin. Conclusion: Taken together, the results of the study indicated that CAPE might exert its anti-neoplastic effect by targeting PLD1 expression in AGS cell line. Keywords: Cancer, Caffeic acid phenethyl ester, Chrysin, PLD1 gene, Propolis

Item Type: Article
Subjects: QU Biochemistry > Cell biology and genetics
Divisions: Reserach Vice-Chancellar Department > Journal of Herbmed Pharmacology
Depositing User: marzieye nazari .
Date Deposited: 09 Dec 2019 08:52
Last Modified: 09 Dec 2019 08:52
URI: http://eprints.skums.ac.ir/id/eprint/8046

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