Cytotoxicity and in vitro antioxidant potential of Quercus Brantii acorn extract and the corresponding fractions

Moradi, M.T. and Karimi, A. and Alidadi, S. and Ghasemi-Dehkordi, P. and Ghaffari-Goosheh, M.S. (2016) Cytotoxicity and in vitro antioxidant potential of Quercus Brantii acorn extract and the corresponding fractions. International Journal of Pharmacognosy and Phytochemical Research, 8 (4). pp. 558-562.

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Abstract

The present study was mainly aimed to evaluate antioxidant activity and cytotoxicity of hydroalcoholic extract and three corresponding fractions of Quercus brantii acorn. A 70% ethyle alcohole extract of the plant were prepared and sequentially partitioned with n-hexane, chloroform, ethylacetate and n-butanol. The antioxidant potential of all these fractions was evaluated by the 2,2 diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity method. Cytotoxic activity was tested against two normal cell lines (African green monkey kidney [Vero] and human dermal fibroblasts [HDFs]) by MTT assay. The results revealed that the n-butanol fraction exhibited the lowest IC50value (6.5±0.6 μg/ml) with the highest antioxidant activity as compared to the other fractions. The IC50values of the chloroform fraction, the n-butanol fraction, the crude extract, and the n-hexane fraction were found to be significant (p<0.05) as compared with butylated hydroxytoluene (BHT). The results of cytotoxicity showed that the chloroform fraction exhibited the highest cytotoxicity toward Vero and HDFs cell lines at concentration of 60.6±23 and 287.8±38 μg/ml, respectively. We conclude that at least, n-butanol fraction of this plant with high phytoconstituents and less toxicity could be a promising source of medicinally important natural compound. Our findings, therefore, suggest that overall the studied extract/fractions exhibit low cytotoxicity on normal cell lines. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All Rights reserved.

Item Type: Article
Additional Information: cited By 3
Uncontrolled Keywords: Antioxidantو Cytotoxicityو Medicinal plantsو Quercus brantii
Subjects: W General medicine- Health professions > Medical Education W.18
QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: Users 1 not found.
Date Deposited: 05 Jul 2017 06:22
Last Modified: 05 Jul 2017 06:22
URI: http://eprints.skums.ac.ir/id/eprint/798

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