Isolation and molecular identification of carotenoid-producing bacteria

Sadat Solouki Nezhad, Razieh and Asaadi, Hanieh and Eshaghi Milasi, Yaser and Yazdansetad, Sajjad (2019) Isolation and molecular identification of carotenoid-producing bacteria. Nova Biologica Reperta, 6 (1).

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Abstract

Abstract. The production of pigments from bacteria is significant due to the low cost, high yield and ease of extract compared with other sources. Carotenoids are one of the most important pigments with antioxidant properties which are the precursor of vitamin A synthesis and have antibody overproduction ability, anti-tumor activity and inhibitory effect on the cardiovascular disease. The present study aimed to isolate and identify carotenoid-producing bacteria by highperformance liquid chromatography (HPLC) analysis of their carotenoid pigments. Twenty soil samples were collected from different regions of Tehran. After serial dilution each sample was cultured on BHI agar medium and incubated at 37°C. The pigment-producing bacteria were selected for further identification and their pigments were extracted bymethanol. The screening was carried out at two levels: i) selection of the strains by visual color inspection, ii) analysis of the pigment extracts by UV-VIS spectroscopy and HPLC. The isolates were identified by phenotypic methods and their 16S rDNA gene was amplified by PCR method and sequenced. Staphylococcus epidermidis, Micrococcus aloeverae, Citricoccus alkalitolerans, Rhodococcus zopfii, Arthrobacter agilis, Dietzia natronolimnaea and Rhodococcus ruber were identified as carotenoid-producing strains. The highest rate of absorption was observed using UV-VIS analysis in Staphylococcus epidermidis and Dietzia natronolimnaea. The comparison of HPLC analysis with the standard β-carotene curve showed that the carotenoids were beta-carotene. Micro-organisms are a potential source in the production of pigments. In this study we introduced two genera of bacteria (Staphylococcus epidermidis and Dietzia natronolimnaea) with carotenoid-producing ability.

Item Type: Article
Uncontrolled Keywords: β-carotene, carotenoids, chromatography, pigment, spectrophotometry
Subjects: WG Cardiovascular System
QU Biochemistry
QW Microbiology and Immunology
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Cardiology
Depositing User: Unnamed user with email nazari@skums.ac.ir
Date Deposited: 13 Jul 2019 10:29
Last Modified: 13 Jul 2019 10:29
URI: http://eprints.skums.ac.ir/id/eprint/7852

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