In silico analysis of transmembrane protein 31 (TMEM31)antigen to design novel multiepitope peptide and DNA cancer vaccines against melanoma

Safavi, A and Kefayat, A and Abiri, A and Mahdevar, E and Behnia, A and Ghahremani, F (2019) In silico analysis of transmembrane protein 31 (TMEM31)antigen to design novel multiepitope peptide and DNA cancer vaccines against melanoma. Molecular Immunology, 112.

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Abstract

Multiepitope cancer vaccines are announcing themselves as the future of melanoma treatment. Herein, high immunogenic regions of transmembrane protein 31 (TMEM31)antigen were selected according to cytotoxic T lymphocytes’ (CTL)epitopes and major histocompatibility complex (MHC)binding affinity through in silico analyses. The 32–62, 77–105, and 125–165 residues of the TMEM31 were selected as the immunodominant fragments. They were linked together by RVRR and HEYGAEALERAG motifs to improve epitopes separation and presentation. In addition, to activate helper T lymphocytes (HTL), Pan HLA DR-binding epitope (PADRE)peptide sequence and tetanus toxin fragment C (TTFrC)were incorporated into the final construct. Also, the Beta-defensin conserved domain was utilized in the final construct as a novel adjuvant for Toll-like receptor 4/myeloid differentiation factor (TLR4-MD)activation. The CTL epitopes, cleavage sites, post-translational modifications, TAP transport efficiency, and B cells epitopes were predicted for the peptide vaccine. The final construct contained multiple CTL and B cell epitopes. In addition, it showed 93.55% and 99.13% population coverage in the world for HLA I and HLA II, respectively. According to these preliminary results, the multiepitope cancer vaccine can be an appropriate choice for further experimental investigations

Item Type: Article
Uncontrolled Keywords: Cancer vaccine, Cancer/testis antigen, In silico, Melanoma, TMEM31
Subjects: QU Biochemistry
QZ pathology-Neoplasms
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Pathology
Depositing User: Unnamed user with email nazari@skums.ac.ir
Date Deposited: 12 Jun 2019 05:55
Last Modified: 12 Jun 2019 05:55
URI: http://eprints.skums.ac.ir/id/eprint/7768

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