Effects of Pioglitazone On the Lipid Profile, Serum Antioxidant Capacity, and UCP1 Gene Expression in Mouse Brown Adipose Tissue

Mahmoudi, Amin and Ghatreh Samani, Keihan and Amini, Seyed Asadollah and Heidarian, Esfandiar (2019) Effects of Pioglitazone On the Lipid Profile, Serum Antioxidant Capacity, and UCP1 Gene Expression in Mouse Brown Adipose Tissue. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 8 (1).

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Abstract

Background: Pioglitazone increases insulin sensitivity and improves glycemic control in type 2 diabetics. In this study, we evaluated the effects of pioglitazone on the uncoupling protein 1 (UCP1) expression in mouse brown adipose tissue (BAT), and on recovery from oxidative stress due to a high-fat diet. Methods: 30 mice were divided into three groups: group 1 received a normal diet, group 2 received a high-fat diet, and group 3 received a high-fat diet plus 30 mg/kg pioglitazone. After treatment, the cholesterol, triglyceride, paraoxonase 1 (PON1), total serum antioxidant capacity (TAC), malondialdehyde (MDA), and specific activity of hepatic catalase were measured. BAT UCP1 expression was evaluated at both the mRNA and protein levels. Results: The weights differed between the groups (p<0.05). Serum MDA was greater and TAC, liver catalase, and PON1 were less than in group 2 than in group 1 (p<0.05). In Serum MDA was less and catalase activity was greater in group 3 than in group 2 (p<0.05). UCP1 gene expression was less in group 2 than in group 1 (p<0.05) but greater than in group 3 (p<0.05). Conclusions: Pioglitazone may have a protective role in high-fat-diet-induced oxidative stress by increasing the antioxidant capacity. Moreover, it can induce weight loss by increasing UCP1 mRNA and protein expression.

Item Type: Article
Uncontrolled Keywords: LEPTIN, WHITE,High-fat diet, MDA, pioglitazone, PON1, TAC, UCP1
Subjects: WH Hemic and Lymphatic System
QU Biochemistry
QZ pathology-Neoplasms
QV pharmacology
Depositing User: Unnamed user with email nazari@skums.ac.ir
Date Deposited: 10 Jun 2019 07:06
Last Modified: 10 Jun 2019 09:51
URI: http://eprints.skums.ac.ir/id/eprint/7723

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