The effect of warming specimens of rapid urease test on its diagnostic accuracY

Nasiri, Jafar and Allahdin, Arshya and Imani, Reza and Kheiri, Soleiman and Khoshdel, Abolfazl (2019) The effect of warming specimens of rapid urease test on its diagnostic accuracY. REVISTA LATINOAMERICANA DE HIPERTENSION, 14 (1).

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Introduction: Helicobacter pylori (H. pylori) is a prevalent cause of dyspepsia, peptic ulcer and gastric cancer in developing countries. Among various diagnostic methods, rapid urease test (RUT) is the ideal test for its diagnosis in patients undergoing endoscopy. Studies have identified the factors causing false negative and positive results. One of these factors is proposed to be the temperature of keeping specimens, but the results have been controversial. We aimed to compare the diagnostic accuracy of RUT specimens warmed at 37 degrees C or kept at room temperature (22-25 degrees C) in different time intervals until 24 hours. Methods: 100 patients with dyspepsia who were indicated for endoscopic examination were selected based on convenience sampling method from patients referring to Endoscopy center of Hajar Hospital during August-September 2006. After recording the demographic and medical history of patients, three biopsy specimens were taken from one portion of the antrum; the first two samples were placed in home-made RUT solution and the third biopsy was placed in formalin solution; one tube was placed in incubator with 37 degrees C and the other at room temperature (22-25 degrees C). Positivity of RUT test at different times was compared to histopathological examination. Results: Infection with Helicobacter pylori was confirmed by histological examination in 66% of patients. After 24 hours, sensitivity of incubated RUT was 71% at 37 degrees C, and 68% at room temperature (P=0.85). Specificity at 37 degrees C was 59% and at room temperature was 76% (P=0.19). Median time to positive tests was 2 hours at 37 degrees C and 3 hours at room temperature, while RUT became positive faster at 37 degrees C (P=0.553). Conclusion: The non-significant difference between groups revealed that warming could not improve the diagnostic accuracy of RUT test, thus, the standard RUT method is recommended.

Item Type: Article
Subjects: W General medicine- Health professions
WJ Urogenital System
QW Microbiology and Immunology
Divisions: Faculty of Medicine
Depositing User: Unnamed user with email
Date Deposited: 10 Jun 2019 06:27
Last Modified: 10 Jun 2019 09:49

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