Pterostilbene Enhances Anticancer Effects of L-asparaginase in Lymphoblastic Leukaemia Cell Line

Rahimnejad, T and Beshkar, P and Bagheri, R and Pourgheysari, B (2019) Pterostilbene Enhances Anticancer Effects of L-asparaginase in Lymphoblastic Leukaemia Cell Line. INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 81 (2).

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Abstract

Antiproliferative and apoptotic effects of pterostilbene were examined in combination with L-asparaginase in Jurkat cell line. Jurkat cells were incubated with different concentrations of pterostilbene alone or in combination with L-asparaginase for 24, 48 and 72 h. Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Induction of apoptosis was measured by annexin-V fluorescein isothiocyanate and the level of active caspase 3 positive cells by intracellular staining and flowcytometry. Decline of cell viability to 50 % was observed at 67.78 +/- 3.88, 60.97 +/- 3.36 and 52.11 +/- 2.50 mu M concentration after 24, 48 and 72 h incubation with pterostilbene, respectively. Pterostilbene at a concentration of 30, 50 and 70 mu M in combination with 0.5 and 0.7 IU/ml L-asparaginase reduced relative cell growth to a significant level. The rate of apoptosis was significantly higher than control at 80 mu M concentration of pterostilbene and a combination of 60 mu M pterostilbene with 0.5 and 0.7 IU/ml L-asparaginase, but not with L-asparaginase alone. The level of caspase 3 positive cells was significantly higher than control at 80 mu M concentration of pterostilbene. Pterostilbene increased antiproliferative and apoptotic effects of L-asparaginase in Jurkat cells. These results suggested that pterostilbene might be a potential anticancer agent in lymphoblastic leukaemia and potentiate the effect of L-asparaginase. Unravelling the mechanism of pterostilbene-induced cell apoptosis in this cell line could help in the development of a targeted therapy

Item Type: Article
Uncontrolled Keywords: CANCER IN-VITRO, INHIBITS PROLIFERATION, DRUG RESISTANCE, CYCLE ARREST, APOPTOSIS, INDUCTION, CARCINOGENESIS, RESVERATROL, CHILDREN, ANTIBODY
Subjects: W General medicine- Health professions
WB Practice of Medicine
WH Hemic and Lymphatic System
QZ pathology-Neoplasms
Divisions: Faculty of Medicine
Depositing User: Unnamed user with email zamani.m@skums.ac.ir
Date Deposited: 10 Jun 2019 04:36
Last Modified: 10 Jun 2019 09:35
URI: http://eprints.skums.ac.ir/id/eprint/7713

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