The effect of some polymorphisms in vitamin D receptor gene in menopausal women with osteoporosis

Dehghan, Morteza. and Pourahmad-Jaktaji, Razieh. (2016) The effect of some polymorphisms in vitamin D receptor gene in menopausal women with osteoporosis. Journal of Clinical and Diagnostic Research, 10 (6). RC06-RC10.


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Introduction: Vitamin D receptor gene is one of candidate genes related to osteoporosis expansion. The association of ApaI, TaqI, and BsmI polymorphisms in vitamin D receptor gene with bone metabolism and density has been already demonstrated. Aim: This study was conducted to investigate the association between the ApaI, TaqI and BsmI polymorphisms and bone density. This study was descriptive-analytical study. Centers for bone density measurement in southwestern Iran. Materials and Methods: In this descriptive-analytical study, 200 participants aged 45- and above 45-year-old women referring the centers of bone density measurement participated. The bone density of femoral neck and lumbar vertebrae was measured using dual-energy X-ray absorptiometry method. Based on t-score, the participants were assigned into patients (n=130) and healthy individuals (n=70). Different genotypes of ApaI (AA/Aa/aa), TaqI (TT/Tt/tt), and BsmI (BB/Bb/bb) were determined by PCR-RFLP. The data on bone density and PCR-RFLP were analysed by chi-square and ANOVA. Also, triad combination of the genotypes was statistically analysed. For each genotype combination, chi-square was run between the patients and control group and p-value was calculated. Results: No significant association was seen between ApaI polymorphism and bone density (p>0.05). TaqI and BsmI polymorphisms had a significant association with femoral neck’s bone density (p<0.05), but these polymorphisms were not significantly associated with lumbar vertebrae’s (p>0.05). Patients with homozygous dominant TT genotype had the least bone density in femoral neck compared to other genotypes. Lumbar vertebrae’s bone density was similar in three TaqI genotypes. The patients with homozygous recessive bb genotype had the least bone density in femoral neck and lumbar vertebrae compared to other genotypes. Conclusion: TaqI and BsmI polymorphisms could be desirable markers in diagnosis of women at risk of osteoporosis in the studied region in Iran. Therefore, these women will receive suitable medical treatment at proper time

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Bone densityو DNA markerو PCR-RFLP
Subjects: WP Gynecology
QU Biochemistry
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Orthopedics
Depositing User: Users 1 not found.
Date Deposited: 04 Jul 2017 09:55
Last Modified: 15 Apr 2018 09:41

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