Transplantation of miR-219 overexpressed human endometrial stem cells encapsulated in fibrin hydrogel in spinal cord injury

Jalali Monfared, M and Nasirinezhad, F and Ebrahimi-Barough, S and Hasanzade, G and Saberi, H and Tavangar, S and Asadpour, S and Aryan, L and Barabadi, Z (2019) Transplantation of miR-219 overexpressed human endometrial stem cells encapsulated in fibrin hydrogel in spinal cord injury. Journal of Cellular Physiology.

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Abstract

Oligodendrocyte (OL) loss and demyelination occur after spinal cord injury (SCI). Stimulation of remyelination through transplantation of myelinating cells may be effective in improving function. For the repair strategy to be successful, the selection of a suitable cell and maintaining cell growth when cells are injected directly to the site of injury is important. In addition to selecting the type of cell, fibrin hydrogel was used as a suitable tissue engineering scaffold for this purpose. To test the relationship between myelination and functional improvement, the human endometrial stem cells (hEnSCs) were differentiated toward oligodendrocyte progenitor cells (OPCs) using overexpression of miR-219. Adult female Wistar rats were used to induce SCI by using a compression model and were randomly assigned to the following four experimental groups: SCI, Vehicle, hEnSC, and OPC. Ten days after injury, miR-219 overexpressed hEnSC-derived OPCs encapsulated in fibrin hydrogel, as an injectable scaffold, were injected to the injury site. In this study, with a focus on promoting functional recovery after SCI, the Basso-Beattie-Bresnahan test was performed to evaluate the recovery of motor function every week for 10 weeks and the histological assay was then performed. Results showed that the rate of motor function recovery was significantly higher in OPC group compared to SCI and vehicle groups but no marked differences were found between OPC and hEnSC groups, although, the rate of myelination in the OPC group was significantly higher than the other groups. These results demonstrated that remyelination was not the cause of recovery of motor function

Item Type: Article
Uncontrolled Keywords: demyelination and remyelination, fibrin hydrogel, human endometrial stem cell, spinal cord injury
Subjects: WB Practice of Medicine
WL Nervous system
QU Biochemistry
QZ pathology-Neoplasms
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Neurological Diseases
Depositing User: Unnamed user with email zamani.m@skums.ac.ir
Date Deposited: 05 May 2019 08:58
Last Modified: 05 May 2019 08:59
URI: http://eprints.skums.ac.ir/id/eprint/7699

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