Inhibition of MicroRNA miR-222 with LNA Inhibitor Can Reduce Cell Proliferation in B Chronic Lymphoblastic Leukemia

Dehkordi, K.A. and Chaleshtori, M.H. and Sharifi, M. and Jalili, A. and Fathi, F. and Roshani, D. and Nikkhoo, B. and Hakhamaneshi, M.S. and Sani, M.R.M. and Ganji-Arjenaki, M. (2016) Inhibition of MicroRNA miR-222 with LNA Inhibitor Can Reduce Cell Proliferation in B Chronic Lymphoblastic Leukemia. Indian Journal of Hematology and Blood Transfusion. pp. 1-6.

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Abstract

MicroRNAs (miRNAs) are small regulatory molecules that negatively regulate gene expression by base-pairing with their target mRNAs. miRNAs have contribute significantly to cancer biology and recent studies have demonstrated the oncogenic or tumor-suppressing role in cancer cells. In many tumors up-regulation miRNAs has been reported especially miR-222 has been shown to be up-regulated in B chronic lymphocytic leukemia (B-CLL). In this study we assessed the effected inhibition of miR-222 in cell viability of B-CLL. We performed inhibition of mir-222 in B-CLL cell line (183-E95) using locked nucleic acid (LNA) antagomir. At different time points after LNA-anti-mir-222 transfection, miR-222 quantitation and cell viability were assessed by qRT-real time polymerase chain reaction and MTT assays. The data were analyzed by independent t test and one way ANOVA. Down-regulation of miR-222 in B-CLL cell line (183-E95) with LNA antagomir decreased cell viability in B-CLL. Cell viability gradually decreased over time as the viability of LNA-anti-mir transfected cells was <47 % of untreated cells at 72 h post-transfection. The difference in cell viability between LNA-anti-miR and control groups was statistically significant (p < 0.042). Based on our findings, the inhibition of miR-222 speculate represent a potential novel therapeutic approach for treatment of B-CLL.

Item Type: Article
Additional Information: cited By 0; Article in Press
Uncontrolled Keywords: Chronic lymphocytic leukemiaو Locked nucleic acidو MicroRNAو miR-222
Subjects: WH Hemic and Lymphatic System
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 04 Jul 2017 08:46
Last Modified: 04 Jul 2017 08:46
URI: http://eprints.skums.ac.ir/id/eprint/763

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