Inhibition of MicroRNA miR-222 with LNA Inhibitor Can Reduce Cell Proliferation in B Chronic Lymphoblastic Leukemia

Ashrafi Dehkordi, Korosh. and Hashemzadeh-Chaleshtori, Morteza. and Sharifi, Mohamadreza. and Jalili, Ali. and Fathi, Fardin and Roshani, Daem. and Nikkhoo, Bahram. and Hakhamaneshi, Mohammad Saeed. and Mahmoodian Sani, Mohammad Reza. and Ganji-Arjenaki, Mahboube (2016) Inhibition of MicroRNA miR-222 with LNA Inhibitor Can Reduce Cell Proliferation in B Chronic Lymphoblastic Leukemia. Indian Journal of Hematology and Blood Transfusion. pp. 1-6.


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MicroRNAs (miRNAs) are small regulatory molecules that negatively regulate gene expression by base-pairing with their target mRNAs. miRNAs have contribute significantly to cancer biology and recent studies have demonstrated the oncogenic or tumor-suppressing role in cancer cells. In many tumors up-regulation miRNAs has been reported especially miR-222 has been shown to be up-regulated in B chronic lymphocytic leukemia (B-CLL). In this study we assessed the effected inhibition of miR-222 in cell viability of B-CLL. We performed inhibition of mir-222 in B-CLL cell line (183-E95) using locked nucleic acid (LNA) antagomir. At different time points after LNA-anti-mir-222 transfection, miR-222 quantitation and cell viability were assessed by qRT-real time polymerase chain reaction and MTT assays. The data were analyzed by independent t test and one way ANOVA. Down-regulation of miR-222 in B-CLL cell line (183-E95) with LNA antagomir decreased cell viability in B-CLL. Cell viability gradually decreased over time as the viability of LNA-anti-mir transfected cells was <47 % of untreated cells at 72 h post-transfection. The difference in cell viability between LNA-anti-miR and control groups was statistically significant (p < 0.042). Based on our findings, the inhibition of miR-222 speculate represent a potential novel therapeutic approach for treatment of B-CLL.

Item Type: Article
Additional Information: cited By 0; Article in Press
Uncontrolled Keywords: Chronic lymphocytic leukemiaو Locked nucleic acidو MicroRNAو miR-222
Subjects: WH Hemic and Lymphatic System
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 04 Jul 2017 08:46
Last Modified: 04 Mar 2018 04:56

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