Frequency assessment of OXA-10 and PER β-lactamase genes and determination of minimum inhibitory concentration in klebsiella strains isolated from urinary tract infections

Kakian, F and Abadi, M and Zamanzad, B and Najafi, H and Amiri, M and Gholipour, A (2019) Frequency assessment of OXA-10 and PER β-lactamase genes and determination of minimum inhibitory concentration in klebsiella strains isolated from urinary tract infections. Jundishapur Journal of Microbiology, 12 (2).

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Abstract

Background: Klebsiella is one of the Enterobacteriaceae family that causes infections such as pneumonia, urinary tract infections (UTI), and meningitis. Klebsiella strains are capable of producing enzymes that can degrade the third-generation of cephalosporins known as broad-spectrum beta-lactamase enzymes. The resistance of Klebsiella strains to β-lactam antibiotics is related to the presence of β-lactamase genes. Methods: In this study, 90isolates of Klebsiella were isolated from two inpatient andoutpatient groups, each of themwas 45 isolates, which were collected from patients with urinary tract infection in educational hospitals of Shahrekord. The isolates were identified using phenotypic agar diffusion, disc phenotypic confirmation tests, and E-test of extended-spectrum beta-lactamase (ESBL). The PCR molecular method was used to diagnose and determine the strains containing broad-spectrum β-lactamases. Results: Thirty (66%) inpatients and 8 (17.8%) outpatients had broad-spectrum β-lactamase enzymes. The frequency of β-lactamase OXA-10 genes and PER in inpatients were 90% and 33%, respectively and also in outpatients were 50% and 12.5%, respectively. Conclusions: This study showed that the prevalence of isolated Klebsiella producing broad-spectrum β-lactamases is higher in inpatients in comparison to outpatients. Therefore, the rapid and accurate identification of bacteria and their resistance genes in clinical microbiology labs are highly recommended

Item Type: Article
Uncontrolled Keywords: Extended Spectrum β-Lactamases, Klebsiella, Minimum Inhibitory Concentration, OXA-10, PER Gene
Subjects: WB Practice of Medicine
WD Nutrition Disease and metabolic diseases
WJ Urogenital System
QZ pathology-Neoplasms
QV pharmacology
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Urology
Depositing User: Unnamed user with email zamani.m@skums.ac.ir
Date Deposited: 06 Apr 2019 09:55
Last Modified: 17 Apr 2019 03:43
URI: http://eprints.skums.ac.ir/id/eprint/7593

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