A Survey on the Adjuvant Role of Naloxone Alone or Combined with Alum in Vaccination Against Fasciolosis in BALB/c Mice.

Azizi, H and Mirzaeei, H and Bagheri, A and Bazi, A and Khamesipour, A and Yaghoobi, H and Mirzapour, A and Khatami, M and Elikaee, S (2019) A Survey on the Adjuvant Role of Naloxone Alone or Combined with Alum in Vaccination Against Fasciolosis in BALB/c Mice. Acta Parasitol.

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Abstract

BACKGROUND: Fasciolosis is a zoonotic parasitic disease imposing a heavy load of livestock losses worldwide. PURPOSE: We aimed to evaluate immune-stimulatory effects of naloxone (NLX), an opioid receptor antagonist, in combination with alum in mice vaccinated with excretory-secretory antigens (E/S) of Fasciola hepatica. METHODS: 8-week-old female BALB/c mice were subcutaneously vaccinated using E/S antigens of F. hepatica. Experimental groups (14 mice per group) included: vaccine (E/S antigen), alum vaccine (E/S antigen plus alum), NLX vaccine (E/S antigen plus NLX), and alum-NLX vaccine (E/S antigen plus a mixture of alum-NLX). The control group was infused with PBS. Lymphocyte proliferation and the levels of IFN-γ, IL-4, IgG2a, IgG1, and total IgG were measured. RESULTS: Mice vaccinated with NLX or alum-NLX adjuvants showed significantly higher rates of lymphocyte proliferation, IFN-γ, total IgG, and IgG2a levels. The mice that were injected with alum showed a significantly higher concentration of IL-4. Ratios of IFN-γ/Il-4 and IgG2a/IgG1 were significantly higher in the NLX and alum-NLX groups in comparison with the groups vaccinated either with alum or without any adjuvant. A significantly higher protection rate (62.5%) was seen in mice vaccinated with the alum-NLX adjuvant compared to the other groups. CONCLUSION: NLX can be effective in conferring cellular immunity and protection against F. hepatica. It is recommended to consider this agent as a potential adjuvant in vaccines against fasciolosis.

Item Type: Article
Uncontrolled Keywords: Adjuvant, Aluminum sulfate, Fasciolosis, Naloxone, Vaccine
Subjects: WC Communicable Diseases
QV pharmacology
QW Microbiology and Immunology
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Community Medicine
Depositing User: Unnamed user with email nazari@skums.ac.ir
Date Deposited: 11 Mar 2019 05:43
Last Modified: 10 Apr 2019 03:59
URI: http://eprints.skums.ac.ir/id/eprint/7567

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