Quantitative gene expression of ERG9 in model Saccharomyces cerevisiae: Chamomile extract for human cancer treatment

Hosseinpour, Maryam. and Mobini-Dehkordi, Mohsen. and Teimori, Hossein. (2016) Quantitative gene expression of ERG9 in model Saccharomyces cerevisiae: Chamomile extract for human cancer treatment. Journal of Clinical and Diagnostic Research, 10 (7). FC05-FC08.

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Abstract

Over expression of squalene synthase gene causes induction of growth tumour and reduction of apoptosis. This gene which is conserved between Saccharomyces cerevisiae yeast and humans, is named (ERG9). Aim: In this work, we studied the effect of Matricaria recutita extract on ERG9 gene (squalene synthase) expression in S.cerevisiae which was used as organism model in cancer therapy. Materials and Methods: S. cerevisiae was cultured in YPD medium plus 0,250, 1000 and 3000 µg/ml of Matricaria recutita extract and we evaluated the (ERG9) gene expression by Realtime RT-PCR method after 24 hours. Statistical analysis used: At least 3 independent experiments were done. Data were analyzed using One-way ANOVA and Dunnett’s test. A p-value of less than 0.01 was considered as significant. Results: We found that 250, 1000 and 3000 µg/ml of Matricaria recutita extract could reduce expression of ERG9 gene significantly (p<0.01). Interestingly, the expression of this gene was completely inhibited in 1000 and 3000 µg/ml concentrations. Conclusion: This study predicted that Matricaria recutita extract produced anti-cancer effects in humans, because it could inhibit the expression of an analogue key gene in this malignant disease. Further investigations should be made, to study its molecular mechanism of action at the mammal cell level.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Hydro alcoholic extractو Matricaria recutitaو Medicinal plants
Subjects: QZ pathology-Neoplasms
QV pharmacology > QV 752 Pharmacognosy
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Biochemistry and Genetics
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 04 Jul 2017 07:04
Last Modified: 09 May 2018 05:44
URI: http://eprints.skums.ac.ir/id/eprint/754

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