In vivo anti-staphylococcal activity of roselle (Hibiscus sabdariffa L.) calyx extract in Drosophila model of infection

Muhammad, Ahsan and Alvin Valentino, Gonsales and Sartini, Sartini and Elly, Wahyudin and Firzan, Nainu (2019) In vivo anti-staphylococcal activity of roselle (Hibiscus sabdariffa L.) calyx extract in Drosophila model of infection. Journal of Herbmed Pharmacology, 8 (1).

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Abstract

Introduction: The emergence of antibiotic-resistant Staphylococcus aureus is a major threat for worldwide communities. To overcome such serious problem, the discovery of novel antibacterial agents through exploration of diverse potential sources is essential. The aim of this research was to investigate the in vivo anti-staphylococcal activity of roselle (Hibiscus sabdariffa L.) calyx extract against S. aureus in fruit flies (Drosophila melanogaster) model of infection. Methods: Roselle calyces were dried and extracted with ethanol using maceration method. Wildtype fruit flies were infected with S. aureus and subjected to survival assay, bacterial load examination, and gene expression analysis, in the presence or absence of roselle calyx extract. Survival and bacterial load analysis were subsequently performed on immunodeficient fruit flies using similar protocols. Results: Reduction of host survivorship accompanied by increasing level of bacterial proliferation was observed in group of wildtype fruit flies infected with S. aureus. These phenotypic events were further augmented in mutant flies devoid of component for antibacterial immune responses. Nevertheless, reduction of bacterial load and improvement of host survival were demonstrated in either wildtype or immunodeficient fruit flies upon treatment with roselle calyx extract after bacterial challenge, irrespective of immune status. Conclusion: Collectively, our results demonstrated the in vivo antibacterial activity of roselle calyx extract against S. aureus in Drosophila model of infection and this was not due to induction of immune response in the host.

Item Type: Article
Uncontrolled Keywords: Antibacterial agent, Staphylococcus aureus,Roselle, Fruit flies
Subjects: WC Communicable Diseases
QV pharmacology
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Infectious Diseases
Depositing User: Unnamed user with email nazari@skums.ac.ir
Date Deposited: 20 Jan 2019 10:43
Last Modified: 17 Apr 2019 04:05
URI: http://eprints.skums.ac.ir/id/eprint/7502

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