Application of Dirofilaria immitis immunoreactive proteins in serodiagnosis

Khanmohammadi, M and Falak, R and Meamar, AR and Razmjou, E and Mokhtarian, K and Arshadi, M and Shayanfar, N and Akhlaghi, L (2019) Application of Dirofilaria immitis immunoreactive proteins in serodiagnosis. Parasite Immunol, 41 (1).

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Dirofilariasis is a zoonotic global vector-borne disease caused by Dirofilaria immitis. The present study focuses on the somatic and excretory/secretory (E/S) proteins released from adult D. immitis. We aimed to fractionate and identify adult D. immitis immunoreactive proteins. Somatic and E/S extracts were immunoblotted to identify the immunoreactive proteins. In the current study, we used matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF/MS) to characterize the immunogenic proteins. Additionally, we used fast protein liquid chromatography (FPLC) to fractionate and evaluate the immunogenicity of the D. immitis secretome. The most immunoreactive proteins were between 10 and 48 kDa. Six proteins including polyprotein antigen, P22u, pepsin inhibitor Dit33, neutrophil chemotactic factor (DiNCF) precursor, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and heat-shock protein 70 (HSP70) were found in both somatic and E/S extracts. Eluting the FPLC column with NaCl resolved two peaks in which the immunoreactivities of the purified proteins were conserved. Characterization of these proteins could provide a novel perspective for understanding the pathogenesis and diagnosing of this disease.

Item Type: Article
Uncontrolled Keywords: Dirofilaria immitis, FPLC, dirofilariasis, mass spectrometry, serodiagnosis
Subjects: QU Biochemistry
QW Microbiology and Immunology
Divisions: Faculty of Medicine
Depositing User: Unnamed user with email
Date Deposited: 24 Dec 2018 10:21
Last Modified: 14 Apr 2019 03:56

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