The effect of resveratrol on expression of matrix metalloproteinase 9 and its tissue inhibitors in vascular smooth muscle cells

Farrokhi, Effat and Ghatreh-Samani, Keihan and Salehi-Vanani, Najmeh and Mahmoodi, Amin (2018) The effect of resveratrol on expression of matrix metalloproteinase 9 and its tissue inhibitors in vascular smooth muscle cells. ARYA ATHEROSCLEROSIS, 14 (4).

[img] Text
ARYA-14-157.pdf

Download (464kB)

Abstract

BACKGROUND: Matrix metalloproteinase 9 (MMP-9) is involved in extracellular matrix degradation and remodeling. An increase in MMP-9 expression by vascular component cells plays an important role in atherosclerotic plaque formation and rupture. Resveratrol, a polyphenolic substance, was suggested to play a role in preventing the progress of atherosclerotic disease. The aim of this study was to investigate the effect of resveratrol on MMP-9 and tissue inhibitors of metalloproteinases (TIMPs) in vascular smooth muscle cells (VSMCs) after treatment with H2O2. METHODS: Cultured VSMCs were pre-treated with 0.2 mM of H2O2 before stimulation with different concentration of resveratrol. Expression of MMP-9, TIMP-1, and TIMP-3 genes were measured using real-time polymerase chain reaction (PCR) method, and MMP-9 protein level was detected using western blot analysis. RESULTS: Resveratrol at 120 mu mol/l concentration reduced the elevated level of MMP-9 induced by H2O2 in VSMCs as 1.85 +/- 0.35 folds (P < 0.05) and 8.70 +/- 1.20 folds (P < 0.05) after 24 and 48 hours, respectively. Resveratrol increased the diminished level of TIMP-1 induced by H2O2 as 2.5 +/- 0.48 folds following the treatment with 120 mu mol/l after 48 hours (P < 0.05). CONCLUSION: Resveratrol as an antioxidant can decrease MMP-9 production, not only by suppressing MMP-9 expression, but also by augmenting TIMP-1 production. Altogether, resveratrol as an antioxidant can regulate the MMP-9/TIMP-1 balance, and may be considered as a preservative agent in the treatment and prevention of atherosclerosis.

Item Type: Article
Uncontrolled Keywords: CEREBRAL-ISCHEMIA-REPERFUSION, ABDOMINAL AORTIC-ANEURYSM, NF-KAPPA-B, MATRIX METALLOPROTEINASES, OXIDATIVE STRESS, DISEASE, TRANSCRIPTION, POLYPHENOLS, DYSFUNCTION, PREVENTION
Subjects: QS Human Anatomy
QS Human Anatomy

QV pharmacology
Divisions: Faculty of Medicine
Depositing User: Unnamed user with email zamani.m@skums.ac.ir
Date Deposited: 01 Dec 2018 10:27
Last Modified: 20 Apr 2019 04:03
URI: http://eprints.skums.ac.ir/id/eprint/7411

Actions (login required)

View Item View Item