Characterization of CD4+and CD8+T Cell Subsets and Interferon Regulatory Factor 4 (IRF4) in MS Patients Treated with Fingolimod (FTY-720): A Follow-up Study

Laribi, Bahareh and Sahraian, Mohammad Ali and Shekarabi, Mehdi and Emamnejad, Rahimeh and Marzban, Mohsen (2018) Characterization of CD4+and CD8+T Cell Subsets and Interferon Regulatory Factor 4 (IRF4) in MS Patients Treated with Fingolimod (FTY-720): A Follow-up Study. IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY, 17 (4).

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Abstract

Fingolimod is a novel immunomodulatory drug used in patients with relapsing multiple sclerosis (MS) which reversibly inhibits egress of lymphocytes from lymph nodes. In this longitudinal study, the frequency of Interferon- gamma (IFN-gamma)+, IL4+, IL17+ and IL10+ CD4+ and CD8+ T cell subsets were measured in Fingolimod treated patients before and after 12 months'(12M) therapy using flow cytometry and compared to those of naive, Betaferon treated MS patients and healthy individuals. Additionally, the level of transcription factor IRF4 and IL-6, IL-23, TGF-beta 1 cytokines, required for differentiation of IL-17+ T cells, were assessed by RT-PCR and ELISA, respectively. In Fingolimod treated MS patients, we observed a significant decrease in the percentage of IFN-gamma+/IL17+ CD4+ and CD8+ T cell subsets. In contrast, Fingolimod increased IL10+ CD4+ T cells. We also showed that IFN-gamma+IL17+ co-producing CD8+ T cells were reduced in patients under fingolimod therapy. furthermore, Fingolimod could reduce the expression level of IRF4 in patients while IL6 was increased in the supernatant of cultured peripheral blood mononuclear cells. Our data showed that Fingolimod treatment alters CD4+ and CD8+ T cell subsets and reduces expression of IRF-4, which affects the proportion of pathogenic memory T cells in peripheral blood.

Item Type: Article
Uncontrolled Keywords: RELAPSING MULTIPLE-SCLEROSIS, HUMAN TH17 CELLS, CD8(+) T-CELLS, TRANSCRIPTION FACTOR, ORAL FINGOLIMOD, TGF-BETA, PERIPHERAL-BLOOD, T-H-17 CELLS, EFFECTOR, FTY720
Subjects: QS Human Anatomy
QS Human Anatomy
university Structure > QS Human Anatomy

QV pharmacology
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: Unnamed user with email nazari@skums.ac.ir
Date Deposited: 13 Nov 2018 06:22
Last Modified: 20 Apr 2019 09:14
URI: http://eprints.skums.ac.ir/id/eprint/7353

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