Radioprotective effects of Silymarin on the sperm arameters of NMRI mice irradiated with γ-rays.

Fatehi, Daryoush and Mohammadi, Mohsen and Shekarchi, Babak and Shabani, Arash and Seify, Mohammad and Rostamzadeh, Ayoob (2018) Radioprotective effects of Silymarin on the sperm arameters of NMRI mice irradiated with γ-rays. J Photochem Photobiol B.


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Free radicals and reactive oxygen species (ROS) are generated using various endogenous systems or from external sources such as exposure to different physiochemicals. Ionizing radiation damage to the cell can be caused by the direct or indirect effects of radiotherapy processes. Silymarin (SM), a flavanolignan compound, has been identified as a natural potent antioxidant with cytoprotection activities due to scavenging free radicals. The aim of the present study was to evaluate the radioprotective effect of SM on sperm parameters of mice induced by γ-rays. A total number of 40 adult, male NMRI mice were randomly divided into four equal groups. The control group was neither treated with SM nor irradiated by γ-rays. The second group was only irradiated with 2Gy of γ-rays. The third group was firstly treated with 50mg/kg of SM for 7 consecutive days, and one day later, last injections were irradiated by 2Gy of γ-rays. The fourth groups received only 50mg/kg of SM for 7 consecutive days. All the animals were treated intraperitoneally. Histopathological and morphometrical examinations were performed. The data were analyzed using ANOVA and Tukey post hoc test. A value of p<0.05 was considered significant. The results showed that in the radiation-only group when compared with those treated with SM and irradiated, a significant different was observed in testicular parameters and DNA damage (p<0.05). In conclusion, SM can be considered as a promising herbal radioprotective agent in complementary medicine which may play an important role to protect normal spermatocytes against possible effects of γ-radiation-induced cellular damage.

Item Type: Article
Uncontrolled Keywords: Antioxidants, Fertility, Radiotherapy, Silymarin, Spermatogenesis, γ-rays
Subjects: WN Radiology . Diagnostic Imaging
WP Gynecology
QV pharmacology
Divisions: Faculty of Medicine
Depositing User: Unnamed user with email
Date Deposited: 17 Sep 2018 08:40
Last Modified: 20 Apr 2019 04:27

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