In vitro anti-herpes simplex virus activity, antioxidant potential and total phenolic compounds of selected iranian medicinal plant extracts

Moradi, M.T. and Karimi, A. and Alidadi, S.B. and Hashemi, L. (2018) In vitro anti-herpes simplex virus activity, antioxidant potential and total phenolic compounds of selected iranian medicinal plant extracts. Indian Journal of Traditional Knowledge, 17 (2).

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Drug resistant strains of herpes simplex virus-1 (HSV-1) have been increased recently. Essentially, medicinal plant-based new antiviral agents that are effective against HSV-1 infections are urgently required. Therefore, this research was conducted to evaluate in vitro anti HSV-1 activity of 25 plant extracts. In this study, the hydroalchoholic extracts of different parts of 25 medicinal plants belonging to 16 different families were prepared. Anti-HSV-1 activity was evaluated on Vero cell line using MTT (3-[4,5-dimethylthiazol–2-yl]-2,5-diphenyltetrazolium bromide) assay. The 50 % effective concentration (EC50) and 50 % cytotoxicity concentration (CC50) of the extract were determined using regression analysis. The inhibitory effect of the plant materials on adsorption and/or post-adsorption stages of HSV-1 replication cycle was determined. Antioxidant activity, total phenolic content, and total flavonoid content of the extracts were determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, Folin-Ciocalteu reagent, and aluminum chloride colorimetric method, respectively. Results showed that Pistacia atlantica Desf., Equisetum arvense L., Melissa officinalis L., Anthyllis vulneraria L., Punica granatum L., Syzygium aromaticum (L.) Merr. & L.M. Perry, Camellia sinensis (L.) Kuntze, and Crataegus azarolus L., were active against HSV-1. There was a significant association of total phenolic contents (R = 0.773, p < 0.001) and the free radical scavenging property (R = -0.684, p < 0.01) with the antiviral activity of the extracts. Some of the Iranian plant extracts studied showed potent antiviral activity against HSV-1 and can be used to develop new and effective anti-HSV-1 agents. © 2018, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.

Item Type: Article
Uncontrolled Keywords: Antioxidant potential, Antiviral activity, Herpes simplex virus
Subjects: WC Communicable Diseases > Infection.Bacterial Infections
QV pharmacology > QV 752 Pharmacognosy
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Clinical Biochemistry Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: Users 1 not found.
Date Deposited: 11 Sep 2018 04:51
Last Modified: 11 Sep 2018 04:51

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