Construction of a recombinant B-cell epitope vaccine based on a der p1-derived hypoallergen: A bioinformatics approach

Fanuel, Songwe. and Tabesh, Saeideh. and Mokhtarian, Kobra. and Saroddiny,, Esmaeil. and Fazlollahi, Mohammad Reza. and Pourpak, Z. and Falak, Reza. and Kardar, Gholam Ali. (2018) Construction of a recombinant B-cell epitope vaccine based on a der p1-derived hypoallergen: A bioinformatics approach. Immunotherapy, 10 (7).

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Abstract

Aim: House dust mite (HDM) allergens are important elicitors of IgE-mediated allergies. This study was aimed at constructing and characterizing a recombinant fusion protein, DpTTDp, which was based on carrier-bound Der p 1-derived peptides for HDM allergen immunotherapy. Methods: Using the Immune Epitope Database (IEDB), we identified from Der p 1, a 34-mer hypoallergenic peptide. Two copies of the hypoallergen were then fused to a partial fragment of a tetanus toxoid molecule's N-and C terminus and expressed in Escherichia coli. After purification to homogeneity, the protein was evaluated for allergenicity and its ability to induce blocking antibodies upon immunization. Results: Upon immunization of mice, DpTTDp induced high levels of protective IgG-antibodies that blocked allergic patients' IgE reactivity to HDM. In addition, DpTTDp lacked relevant IgE-reactivity, induced low T-cell proliferation and IFN-γ in peripheral blood mononuclear cells of HDM-allergic patients' sera. Conclusion: The protein represents a promising HDM-allergy immunotherapy candidate vaccine. © 2018 Future Medicine Ltd.

Item Type: Article
Uncontrolled Keywords: allergen-specific immunotherapy, allergy,Der p1, epitopes, house dust mite,tetanus toxoid
Subjects: WF Respiratory System
QU Biochemistry
QW Microbiology and Immunology
Divisions: Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 28 Aug 2018 08:13
Last Modified: 28 Aug 2018 08:13
URI: http://eprints.skums.ac.ir/id/eprint/7040

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