Suicide gene therapy for breast cancer with a suicide-inducing vector carrying the mammaglobin-1 promoter

Ghanbariasad, Ali. and Bandehpour, M. and Yaghoobi, H. and Kazemi, Bahram. (2018) Suicide gene therapy for breast cancer with a suicide-inducing vector carrying the mammaglobin-1 promoter. Minerva Biotecnologica, 30 (2).

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Abstract

BACKGROUND: Breast carcinoma is the most frequent cancer among women and finding a therapeutic approach is necessary for the treatment of this carcinoma. Gene therapy is a promising therapeutic approach for cancer. Targeted expression of the desired therapeutic proteins within the tumor cells is the best approach to reduce toxicity and improve survival. The mammaglobin-1 gene encodes a novel, breast cancer-associated glycoprotein. Mammaglobin-1 expression is restricted to the mammary gland and the function of the mammaglobin-1 protein is unknown. No mammaglobin-1 expression has been reported in various types of benign tissue or neoplasia other than the breast carcinomas. Over expression of mammaglobin-1 gene was reported in breast cancer tissues by some scientists. In this study, for the first time, the mammaglobin-1 promoter was introduced as a cancer specific promoter with a high efficacy. METHODS: A suicide-inducing vector that expresses the BAX suicide gene under the transcriptional control of the mammaglobin-1 promoter was constructed and evaluated against breast cancer in vitro. RESULT S: These data show that the mammaglobin-1 promoter is active in the breast cancer cell lines. Under the control of the mammaglobin-1 promoter, BAX expression in MD-MBA 438 cell line was five-fold higher than in human embryonic kidney (HEK) cell line and no expression was found in A549 cell line. With the help of mammaglobin-1 promoter, a wonderful enhancement was observed in the apoptosis in breast cancer cell lines. CONCLUSIONS: Concerning the expression of BAX gene under the control of mammaglobin-1, it can be used to specify suicide gene therapy in the treatment of breast tumor. © 2017 EDIZIONI MINERVA MEDICA.

Item Type: Article
Uncontrolled Keywords: Bax protein,Genetic therapy, Human, Mammaglobin A, Neoplasms
Subjects: WP Gynecology
QU Biochemistry > Cell biology and genetics
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 28 Aug 2018 06:41
Last Modified: 28 Aug 2018 06:41
URI: http://eprints.skums.ac.ir/id/eprint/7037

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