Up-regulated Th17 cell function is associated with increased peptic ulcer disease in Helicobacter pylori-infection

Bagheri, Nader. and Razavi, Alireza. and Pourgheysari, Batoul. and Azadegan-Dehkordi, Fatemeh. and Rahimian, Ghorbanali. and Pirayesh, Ashkan. and Shafigh, Mohammad Hadi. and Rafieian-Kopaei, Mahmoud. and Fereidani, Rana. and Tahmasbi, Kamran. and shirzad, Hedayatollah. (2018) Up-regulated Th17 cell function is associated with increased peptic ulcer disease in Helicobacter pylori-infection. Infection, Genetics and Evolution., 60.

[img]
Preview
Text
3.pdf

Download (1MB) | Preview

Abstract

Background: During Helicobacter pylori (H. pylori) infection CD4+ T cells in the gastric lamina propria are hyporesponsive and polarized by Th1/Th17 cell responses controlled by Treg cells. The objective of this study was to determine the number of Th17 cells in gastric mucosa of patients with gastritis and peptic ulcer and determined the relationship between main virulence factor of H. pylori and Th17 cells. Methods and materials: A total of 89 H. pylori-infected gastritis patients, 63 H. pylori-infected peptic ulcer patients and 48 H. pylori-negative non-ulcer dysplasia patients were enrolled in this study. The number of Th17 was determined by immunohistochemistry. IL-8 and IL-17A expressions were determined by real-time polymerase chain reaction (qPCR). Also, the grade of chronic and active inflammation was investigated for involvement according to the density of neutrophils and mononuclear in gastric mucosal crypts, from one to all crypts. Results: The number of Th17 cells and the expression of IL-8 and IL-17A in infected patients were significantly higher than uninfected subjects. The number of Th17 cells and the expression of IL-8 and IL-17A in infected patients with peptic ulcer were significantly higher than patients with gastritis. Additionally, the numbers of Th17 cells as well as the expression of IL-8 and IL-17A were positively correlated with the degree of H. pylori density in infected patients with peptic ulcer, while this correlation was negative in infected patients with gastritis. The numbers of Th17 cells as well as the expression of IL-8 and IL-17A were positively correlated with the degree of chronic inflammation. Conclusion: The predominant Th17 cell responses may play a role in the pathogenesis of peptic ulcers disease in infected patients.

Item Type: Article
Uncontrolled Keywords: Helicobacter pylori,Th17,Gastritis,Peptic ulcer disease,Virulence factor
Subjects: WC Communicable Diseases > Infection.Bacterial Infections
WI Digestive System
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Internal Medicine
Faculty of Medicine > Department of Clinical Sciences > Department of Pathology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: Users 1 not found.
Date Deposited: 28 Aug 2018 05:57
Last Modified: 28 Aug 2018 05:57
URI: http://eprints.skums.ac.ir/id/eprint/7032

Actions (login required)

View Item View Item