Analysis of the expression of mir-34a, mir-199a, mir-30c and mir-19a in peripheral blood CD4+T lymphocytes of relapsing-remitting multiple sclerosis patients

Ghadiri, Nooshin. and Emamnia, Negaralsadat. and Ganjalikhani-Hakemi, Mazdak. and Ghaedie, Kamran. and Etemadifar, Masoud. and Salehi, Mansour. and shirzad, Hedayatollah. and Nasr-Esfahani, Mohammad Hossein. (2018) Analysis of the expression of mir-34a, mir-199a, mir-30c and mir-19a in peripheral blood CD4+T lymphocytes of relapsing-remitting multiple sclerosis patients. Gene, 659.

[img] Text
9.pdf

Download (1MB)

Abstract

Background: Multiple sclerosis is an immune-mediated inflammatory disease of central nervous system. MicroRNAs play important roles in autoimmune diseases such as MS. Objectives: The aim was to evaluate the expression pattern of miR-34a, miR-199a, miR-30c and miR-19a in peripheral blood derived CD4+ T lymphocytes of both relapsing and remitting phases of MS. Methods: Blood samples from 40 RRMS patients (20 in relapsing and 20 in remitting phase) and 20 healthy volunteers were taken. CD4+ T cells were isolated. The expression level of miR-34a, miR-199a, miR-30c and miR-19a, and the percentage of Th17 and Treg cells were measured. Expression of master transcription factors of Th17 and Treg cells and several targets of these miRNAs were also evaluated. Results: Data indicated an increased expression of miR-34a, miR-30c and miR-19a in relapsing phase and decreased expression of miR-199a in remitting phase. ROC curve data add other prestigious information of miR34a, miR-199a, miR-30c and miR-19a by defining relapsing and remitting phase and also healthy cases with high specificity and sensitivity at a proposed optimum cut-off point. Conclusion: Collectively, we showed a correlation between the four miRNAs with different phases of MS and their possible involvement in differentiation pathways of Th17 cells, as the most important players in MS.

Item Type: Article
Uncontrolled Keywords: miR-199,mir-19amiR-30c,miR-34a,Multiple sclerosis,Th17
Subjects: WL Nervous system
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Depositing User: zahra bagheri .
Date Deposited: 27 Aug 2018 07:48
Last Modified: 27 Aug 2018 07:48
URI: http://eprints.skums.ac.ir/id/eprint/7029

Actions (login required)

View Item View Item