The expression analysis of Fra-1 gene and IL-11 protein in Iranian patients with ulcerative colitis

Sabzevary-Ghahfarokhi, Milad. and Shohan, Mojtaba. and shirzad, Hedayatollah. and Rahimian, Ghorbanali. and Bagheri, Nader. and Soltani, Amin. and Deris, Fatemeh. and Ghatreh samani, Mahdi. and Razmara, Ehsan. (2018) The expression analysis of Fra-1 gene and IL-11 protein in Iranian patients with ulcerative colitis. BMC Immunology, 19 (1).

[img]
Preview
Text
8.pdf

Download (1MB) | Preview

Abstract

Abstract Background: Fra-1 (fosl1) belongs to the activator protein1 (AP-1) family inducing IL-11 expression in oxidative stress condition. IL-11 plays a pivotal role in protecting epithelial barriers integrity. In this study, we investigated the Fra-1 gene expression in the inflamed mucosa of patients with ulcerative colitis (UC) as well as its relation to IL-11 expression. Materials and methods: We enrolled 20 patients and 20 healthy controls with definite UC based on the clinical criteria. Fra-1 gene expression in inflamed and non-inflamed colonic biopsies was determined by real-time polymerase chain reaction (RT-PCR). The IL-11 protein concentration was measured by Enzyme-Linked Immunosorbent Assay (ELISA) method. Pearson correlation was applied to calculate the relation between Fra-1 and IL-11. Results: An increased level of Fra-1 gene expression was observed in patients with mild ulcerative colitis. The protein concentration of IL-11 was also increased in mild UC patients. Conversely, a significant decrease of IL-11 protein level was detected in severe UC patients compared to control group. Conclusion: Oxidative stress in inflamed intestinal biopsies can induce fra-1 gene expression. Our findings suggest that Fra-1 transcription factor leads to the production of IL-11 protein in UC patients.

Item Type: Article
Uncontrolled Keywords: Oxidative stress, Ulcerative colitis (UC), Fra-1 gene, IL-11
Subjects: WI Digestive System
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Health > Department of Epidemiology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Microbiology
Faculty of Medicine > Department of Clinical Sciences > Department of Internal Medicine
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: Users 1 not found.
Date Deposited: 27 Aug 2018 07:29
Last Modified: 27 Aug 2018 07:29
URI: http://eprints.skums.ac.ir/id/eprint/7028

Actions (login required)

View Item View Item