The regulatory role of Nrf2 in antioxidants phase2 enzymes and IL-17A expression in patients with ulcerative colitis

Sabzevary-Ghahfarokhi, Milad. and Shohan, Mojtaba. and shirzad, Hedayatollah. and Rahimian, Ghorbanali. and Soltani, Amin. and Ghatreh samani, Mahdi. and Deris, Fatemeh. and Bagheri, Nader. and Shafigh, Mohammedhadi. and Tahmasbi, Kamran. (2018) The regulatory role of Nrf2 in antioxidants phase2 enzymes and IL-17A expression in patients with ulcerative colitis. Pathology - Research and Practice, 214.

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Abstract

Background: Reactive oxygen species (ROS) is one of the pathogenic factors responsible for intestinal injury in Ulcerative colitis (UC). Nuclear factor erythroid-2 related factor 2 (Nrf2) plays a critical role against ROS factors to conserve epithelial integrity. This study aimed to localize Nrf2 and IL-17A protein in the inflamed mucosa of patients with ulcerative colitis. The gene expression of Nrf2 was also correlated with GST-A4 and PRDX1. Materials and methods: A total of 20 patients and 20 healthy controls with definite UC based on the clinical criteria were enrolled for this study. The expression pattern of Nrf2 and IL-17A protein was compared in inflamed and non-inflamed colonic biopsies by immunohistochemical staining. Nrf2, GST-A4 and PRDX1 gene expression were determined by real-time polymerase chain reaction (RT-PCR). Results: In inflamed colonic biopsies, an increased level of Nrf2 protein factor was detected in epithelial cells. Conversely, IL-17A protein was presented more in mononuclear cells in mucosa and lamina propria regions. A significant increase of Nrf2, GST-A4 gene expression was observed in both mild and severe patients with ulcerative colitis. GST-A4 gene expression indicated a high exponential rate in logistic regression. Conclusion: Oxidative stress in inflamed colonic tissue can induce Nrf2 gene expression. The performance of Nrf2 transcription factor may lead to the induction of GST-A4 and PRDX1. IL-17A is less detected in intestinal inflammation, presenting Nrf2 factor. The present findings suggest that Nrf2 function in the gut plays a role in arresting both inflammatory response and oxidative damages of UC.

Item Type: Article
Uncontrolled Keywords: Oxidative stress,Ulcerative colitis,Nrf2,GST-A4,PRDX1
Subjects: WI Digestive System
QU Biochemistry
Divisions: Faculty of Health > Department of Epidemiology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Immunology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Microbiology
Faculty of Medicine > Department of Clinical Sciences > Department of Internal Medicine
Faculty of Medicine > Department of Clinical Sciences > Department of Pathology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: Users 1 not found.
Date Deposited: 26 Aug 2018 06:20
Last Modified: 26 Aug 2018 06:20
URI: http://eprints.skums.ac.ir/id/eprint/7013

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