Yektadoost, Elham. and Falahati, Mojtaba. and Sari, Soyar. and Attar, Farnoosh. (2018) Investigating the in vitro toxicity of silica oxide nanoparticles on differentiated neuronal cells (PC12). jskums., 20 (2).
|
Text
5.pdf Download (576kB) | Preview |
Abstract
Background and aims: Silica oxide nanoparticles (SiO2-NPs) have attracted a great interest in biotechnology and medicinal fields. It has been documented SiO2-NPs can induce cytotoxicity in normal cells. However, until now, the cytotoxicity of SiO2-NPs against nervous system cells did not examine. Methods: In this in vitro study, the activities of caspase-8 and 6 were analyzed in the PC12 cells treated with different concentrations of silica oxide nanoparticles, and was assessed by Elisa plate reader. Data were analyzed using SPSS software, and by One-way ANOVA and Student t-test. Results: The results showed that the caspase-8 and 6 activities increased in a concentration dependent manner. Indeed, the activity of caspase-8 and 6 was induced after exposure to 1-100 µM of SiO2-NPs. However, the SiO2-NPs showed only slight toxicity in the concentration of 0.1 µM (P<0.05). Conclusion: A mitochondrial-dependent pathway activated by caspase-8 and 6 mediated by SiO2-NPs may be involved in apoptosis induced by NPs, and therefore, cell cytotoxicity plays a role in PC12 cells.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | Silica nanoparticles, Cytotoxicity, Apoptosis, Caspase |
Subjects: | QU Biochemistry > Cell biology and genetics QV pharmacology > QV 600 Toxicology |
Divisions: | Reserach Vice-Chancellar Department > Journal of Shahrekord University of Medical Sciences |
Depositing User: | Users 1 not found. |
Date Deposited: | 27 Jun 2018 04:54 |
Last Modified: | 27 Jun 2018 04:54 |
URI: | http://eprints.skums.ac.ir/id/eprint/6891 |
Actions (login required)
![]() |
View Item |