Investigating the in vitro toxicity of silica oxide nanoparticles on differentiated neuronal cells (PC12)

Yektadoost, Elham. and Falahati, Mojtaba. and Sari, Soyar. and Attar, Farnoosh. (2018) Investigating the in vitro toxicity of silica oxide nanoparticles on differentiated neuronal cells (PC12). jskums., 20 (2).

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Abstract

Background and aims: Silica oxide nanoparticles (SiO2-NPs) have attracted a great interest in biotechnology and medicinal fields. It has been documented SiO2-NPs can induce cytotoxicity in normal cells. However, until now, the cytotoxicity of SiO2-NPs against nervous system cells did not examine. Methods: In this in vitro study, the activities of caspase-8 and 6 were analyzed in the PC12 cells treated with different concentrations of silica oxide nanoparticles, and was assessed by Elisa plate reader. Data were analyzed using SPSS software, and by One-way ANOVA and Student t-test. Results: The results showed that the caspase-8 and 6 activities increased in a concentration dependent manner. Indeed, the activity of caspase-8 and 6 was induced after exposure to 1-100 µM of SiO2-NPs. However, the SiO2-NPs showed only slight toxicity in the concentration of 0.1 µM (P<0.05). Conclusion: A mitochondrial-dependent pathway activated by caspase-8 and 6 mediated by SiO2-NPs may be involved in apoptosis induced by NPs, and therefore, cell cytotoxicity plays a role in PC12 cells.

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