Efficacy of Myrtus communis L. to inactivate the hydatid cyst protoscoleces

Mahmoudvand, Hossein. and Fallahi, Shirzad. and Mahmoudvand, Hormoz. and Shakibaie, Mojtaba. and Fasihi Harandi, Majid. and Saedi Dezaki, Ebrahim. (2016) Efficacy of Myrtus communis L. to inactivate the hydatid cyst protoscoleces. Journal of Investigative Surgery, 29 (3).


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Purpose: The present study aims to investigate the scolicidal effects of Myrtus communis L. essential oil against protoscoleces of hydatid cysts and also its toxicity in mice model. Materials and Methods: Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (12.5–100 μl/ml) were used for 5–30 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Moreover, 48 male NMRI mice were used to determine the acute and sub-acute toxicity of M. communis essential oil. One-way ANOVA with Tukey’s post-hoc test was used to assess differences between experimental groups. Results: Findings of the present study demonstrated that the M. communis essential oil at the concentration of 100 μl/ml after 5 min of exposure killed 100% protoscoleces. Similarly, the mean mortality rate of protoscoleces after 10 min of exposure to concentration of 50 μl/ml was 100%. However, lower concentrations (12.5 and 25 μl/ml) of M. communis essential oil provoked a delayed protoscolicidal effects. The LD50 values of intraperitoneal injection of the M. communis essential oil was 2.23 mL/kg body wt. No significant difference (p > .05) was observed in the clinical chemistry and hematological parameters following oral administrations of M. communis essential oil at the doses 0.05, 0.1, 0.2, and 0.4 mL/kg for 14 days. Conclusion: The results showed potent scolicidal activity of M. communis with no significant toxicity, which might be used as a natural scolicidal agent in hydatid cyst surgery.

Item Type: Article
Uncontrolled Keywords: scolicidal effects; myrtle; cystic echinococcosis; hydatid cyst; toxicity
Subjects: QV pharmacology > QV 752 Pharmacognosy
QX Parasitology
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Parasitology and Mycology
Depositing User: Users 1 not found.
Date Deposited: 14 Jan 2018 09:26
Last Modified: 11 Jun 2018 08:16
URI: http://eprints.skums.ac.ir/id/eprint/6829

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