Association of the p53 codon 72 polymorphism with breast cancer in central part of Iran

Rouhi Boroujeni, Hojjat. and Karimi, Mehrdad. and Moshkelani, Saadat. and Parsaei, Pouya. (2013) Association of the p53 codon 72 polymorphism with breast cancer in central part of Iran. African Journal of Pharmacy and Pharmacology, 7 (7).

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Abstract

The tumor suppressor gene protein 53 (p53) plays a general role in cell cycle control, the initiation of apoptosis and in DNA repair. The human p53 gene is mutated and accumulated in more than 50% of cancers. Codon 72 exon 4 polymorphism (Arg72Pro) of the p53 gene has been implicated in cancer risk. This study was aimed at investigating the possible association between p53 Arg72Pro polymorphism and susceptibility to breast cancer among Iranian population. The p53 Arg72Pro genotypes were determined by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis in 135 breast cancer cases and 150 controls. The PCR products were digested with BstU I restriction enzyme and the DNA fragments were then resolved by electrophoresis in 2% agarose gel. Out of the 135 breast cancer samples, 102 (75.55 %) samples were heterozygous (Arg/Pro), 27 (20%) samples homozygous for arginine (Arg/Arg) and 6 (4.45%) samples homozygous for proline (Pro/Pro). The frequencies of the three p53 genotypes; Arg/Pro, Arg/Arg and Pro/Pro in controls were 62, 24 and 14%, respectively. Heterozygosity for Arg/Pro of p53 codon 72 is potentially one of the genetic risk factors for breast cancer. The p53 Arg72Pro polymorphism may be used as a stratification marker in screening individuals at a high risk of breast cancer.

Item Type: Article
Uncontrolled Keywords: Breast cancer, protein 53 (p53), polymorphism, codon 72.
Subjects: WP Gynecology
QZ pathology-Neoplasms
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Surgery
Faculty of Medicine > Department of Clinical Sciences > Department of pharmacology
Depositing User: zahra bagheri .
Date Deposited: 26 Dec 2017 06:11
Last Modified: 15 Apr 2018 04:15
URI: http://eprints.skums.ac.ir/id/eprint/6722

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