PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran

Khademvatan, Sh. and Rahim, F. and Tavalla, M. and Abdizadeh, R. and Hashemitabar, M. (2013) PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran. PloS one, 8 (6).

[img] Other (5)
5.PDF

Download (3MB)

Abstract

Abstract Feces of stray cat are potential sources of gastrointestinal parasites and play a crucial role in spreading and transmitting parasite eggs, larvae, and oocysts through contamination of soil, food, or water. In this study, we investigated the prevalence of Toxocara spp. infection in stray cats in Ahvaz city, southwest Iran. Eggs of Toxocara spp. in feces of stray cats were detected by the sucrose flotation method, and identification was conducted by polymerase chain reaction (PCR) and DNA sequencing. Of the 140 fecal samples that were randomly collected from public environments during the months of January to May 2012, 45% were found to harbour Toxocara spp. eggs. The highest prevalence of Toxocara spp. eggs was found in the central area of Ahvaz city (28.6%). T. canis eggs were found in 4 (6.34%) of the 63 positive samples. Stray cats are found in parks, playgrounds, and other public places and may be a potential contamination risk. Identification of Toxocara spp. using molecular methods is sufficiently sensitive to detect low levels of parasites and identify the different Toxocara spp. in feces. The relatively high prevalence of Toxocara spp. infection may continue to increase due to lack of effective environmental hygiene control in Iran. Consequently, there is a need to plan adequate programs to detect, identify, and control this infection as well as stray cats in the region.

Item Type: Article
Subjects: WA Public Health > Public Health Education WA.18 > WA 30 Social, Economic, and Environmental factor in public health
WA Public Health > WA 30 Social, Economic, and Environmental factor in public health

WC Communicable Diseases > Infection.Bacterial Infections
QX Parasitology
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Parasitology and Mycology
Depositing User: zahra bagheri .
Date Deposited: 16 Dec 2017 05:52
Last Modified: 16 Dec 2017 05:52
URI: http://eprints.skums.ac.ir/id/eprint/6618

Actions (login required)

View Item View Item