Detection of Exotoxins and Antimicrobial Susceptibility Pattern in Clinical Pseudomonas Aeruginosa Isolates

Malek Mohamad, Somayeh. and Rostami, Soodabeh. and Zamanzad, Behnam. and Gholipour, Abolfazl. and Deris, Fatemeh. (2017) Detection of Exotoxins and Antimicrobial Susceptibility Pattern in Clinical Pseudomonas Aeruginosa Isolates. Avicenna Journal of Clinical Microbiology and Infection.

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Abstract

Background: Pseudomonas aeruginosa is a common opportunistic pathogen that causes nosocomial infection in immunocompromised patients. Among different virulence factors, the type III secretion system (TTSS) is an important agent in virulence and development of antimicrobial resistance in P. aeruginosa. Objectives: Previous studies have shown that production of type III secretion system proteins was correlated with increasing virulence and resistance to several antibiotics. In this study we determined the exotoxins genes (exoU and exoS) and pattern of antimicrobial susceptibility in clinical P. aeruginosa isolates. Methods: A total of 175 P. aeruginosa isolates were collected from patients hospitalized in educational hospitals of Shahrekord and Chamran hospital of Isfahan, Iran from April to December 2015. Antimicrobial susceptibility test was performed by disk diffusion test. The presence of exotoxins genes was detected using multiplex PCR of exoU and exoS genes. Results: The antibiotic resistance rate was higher than 70% to many antibiotics. The highest rate of resistance was related to Levofloxacin and Meropenem (155 (88.6%), 148 (84.6%)) respectively. The exoU gene was found in 75 (42.9%) isolates and 136 (77.7%) of the isolates carried the exoS. In addition, 36(20.6%) of the isolates carried the both of gens. A statistical significance was detected between the presence of exoU gene and resistance to pipracillin (P = 0.01). Conclusions: The result of this study was indicated a high resistance rate to the most antibiotic classes and a specific relationship between the virulence genotype and antimicrobial resistance especially more virulent genotype of exoU+ . In order to prevent the spread of more virulent strains in health care facilities, molecular methods alongside antimicrobial susceptibility tests is suggested.

Item Type: Article
Uncontrolled Keywords: Virulence Factors, Genotype, Type III Secretion Systems, Pseudomonas Aeruginosa
Subjects: WC Communicable Diseases > Infection.Bacterial Infections
QW Microbiology and Immunology
Divisions: Faculty of Health > Department of Epidemiology
Faculty of Medicine > Basic Sciences Academic Groups > Department of Microbiology
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: Users 1 not found.
Date Deposited: 13 Dec 2017 06:35
Last Modified: 06 Feb 2018 10:19
URI: http://eprints.skums.ac.ir/id/eprint/6613

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