Nitric oxide metabolite levels during the ectopic osteoinduction in rats

Bigham, A.S. and Shadkhast, M. and Hassanpour, H. and Lakzian, A. and Khalegi, M.R. and Asgharzadeh, Samira. (2009) Nitric oxide metabolite levels during the ectopic osteoinduction in rats. Comparative clinical pathology, 18 (4).

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Abstract

Abstract Nitric oxide (NO) is a cell-signaling molecule that has diverse biological functions. Recent evidence suggests that its production may regulate the metabolism of the osteoblasts and osteoclasts. The aim of this study was to evaluate levels of nitrite and nitrates (NO metabolites) during ectopic osteoinduction in rats. Eighteen male Sprague–Dawley rats (body weight 200–300 g) were used in this study. All animals were anesthetized and the right and left flank areas were shaved, and under aseptic conditions, a muscular pouch was created in each flank: the left was filled with 20 mg of demineralized bone matrix and the right remained empty (sham). Radiographs were taken at 2, 4, and 6 weeks after surgery to trace the ectopic bone formation and muscle mineralization. Blood samples were taken before (as baseline values) and at 2, 4, and 6 weeks after surgery. The mean values of NO metabolites after 6 weeks were significantly higher (p<0.05) than baseline data and at 2 weeks post-surgery. Results from this study indicate that the ectopic osteoinduction caused increased activity of the osteoblasts which subsequently caused increased serum levels of NO metabolites (nitrites and nitrates).

Item Type: Article
Uncontrolled Keywords: Nitric oxide . Ectopic osteoinduction . DBM . Rat
Subjects: QU Biochemistry
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Basic Sciences Academic Groups > Department of Anatomy (Histology and Embryology
Faculty of Medicine > Department of Clinical Sciences > Department of Radiology
Faculty of Medicine > Department of Clinical Sciences > Department of Surgery
Depositing User: Users 1 not found.
Date Deposited: 10 Dec 2017 08:05
Last Modified: 23 May 2018 06:42
URI: http://eprints.skums.ac.ir/id/eprint/6525

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