Effect of Adenosine Receptor Agonist and Ascorbic Acid on Ultrastructure of Hippocampal CA1 Neurons after Ischemia-Reperfusion Injury

Zamani, Mohammad. and Soleimani, Mansooreh. and Alizadeh, Akram. and Katebi, Majid. (2017) Effect of Adenosine Receptor Agonist and Ascorbic Acid on Ultrastructure of Hippocampal CA1 Neurons after Ischemia-Reperfusion Injury. Journal of Isfahan Medical School, 35 (439).

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Abstract

Background: In brain ischemia, blood and oxygen supply decrease and after reperfusion, free radicals and reactive oxygen species (ROS) cause severe damage. As hippocampal injury after ischemia-reperfusion causes some complications, in this study we analyzed the effect of adenosine receptor agonist (N6-cyclopentyladenosine or CPA) and ascorbic acid on ultrastructure of hippocampal CA1 neurons after ischemia-reperfusion. Methods: 35 male rats in 5 groups were used. Ischemia-reperfusion performed by occlusion of common carotids for 15 minutes. CPA and ascorbic acids were intraperitoneally injected for 7 days after ischemia, and 2 weeks before and for 7 days after ischemia, respectively. After 20 days, brain samples were isolated, prepared, and assayed using transmission electron microscopy (TEM). Findings: Ultrastructure assay of hippocampal CA1 neurons after ischemia-reperfusion with transmission electron microscopy showed recovery of intracellular organelles particularly mitochondria of treated groups. In combination therapy, these improvements were better. Conclusion: Intraperitoneal injection of CPA and ascorbic acid after ischemia-reperfusion can reduce neural damage in CA1 region of hippocampus

Item Type: Article
Uncontrolled Keywords: Ischemia, CA1 region, Hippocampal, N6-cyclopentyladenosine, Ascorbic acid, Ultrastructure
Subjects: WG Cardiovascular System
QU Biochemistry
QU Biochemistry > Cell biology and genetics
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: Users 1 not found.
Date Deposited: 28 Nov 2017 09:46
Last Modified: 07 Feb 2018 04:48
URI: http://eprints.skums.ac.ir/id/eprint/6459

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