Determination and comparison rate of expression markers of osteoblast derived of Adipose derived stem cells markers in monolayer and pellet culture models

Hashemibeni, Batool. and Sadeghi, Farzaneh. and Bahrambeigi, Vahid. and Sanaei, Masumeh. and Sharifi, Esmaeel. and Valiani, Ali. and Ghorbani, Masoud. (2015) Determination and comparison rate of expression markers of osteoblast derived of Adipose derived stem cells markers in monolayer and pellet culture models. International Journal of Engineering Research and Development, 11 (1).

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Abstract

Abstract: Nowadays high accident rates, fractures leading to permanent bone disorders and the impossibility of bone transplant have made scientists to look for new methods of repairing injured bones. Considering the application of stem cells in bone tissue engineering, there exists the necessity to investigate various culture methods and suitable fields and scaffolds. Thus, we decided to induce adipose-derived stem cells into osteoblast cells in two systems of pellet culture and monolayer and compare osteogenic markers. Methods: Stem cells have been separated via mechanical and enzymatic methods and cultured in monolayer and pellet culture models with osteogenic medium. Then, RNA was separated from differentiated cells, complementary DNA (cDNA) was synthesized and amplified. Polymerase chain reaction (PCR) product was transferred to electrophoresis gel. The intensity of the bands was measured by Image-J software and analyzed by SPSS. Results: average osteopontin, osteocalcin and Runx2 genes in differentiated cells in the two culture systems showed a significant difference. The expression of osteocalcin, osteopontin and Runx2 gense in pellet system were more than monolayer systems in 21 days. Conclusion: This study indicated that pellet and monolayer culture systems are appropriate for bone engineering but osteocalcin, osteopontin and Runx2 genes expressions were different in the two culture system.

Item Type: Article
Uncontrolled Keywords: - Adipose-derived stem cells, Osteocalcin, Runx2, Osteopontin, Pellet culture, Monolayer
Subjects: WE Musculoskeletal system
QT physiology > physics.mathematics.engineering
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: Users 1 not found.
Date Deposited: 22 Nov 2017 09:58
Last Modified: 07 May 2018 09:31
URI: http://eprints.skums.ac.ir/id/eprint/6408

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