dy of P53 gene mutations in promoter and exons 2-4 and 9-11 in patient with gastric cancer by PCR-SSCP in Chaharmahal Va Bakhtiari province

Saffari Chaleshtary, Javad. and Moradi, Mohammad-Taghi. and Farrokhi, Effat. and Tabatabaiefar, Mohammad Amin. and Taherzadeh Ghahfarrokhi, Maryam. and Azadegan-Dehkordi, Fatemeh. and Mobini, Gholam Reza. and Banitalebi, Mehdi. and Rahimian, Ghorbanali. and Hashemzadeh Chaleshtori, M. (2012) dy of P53 gene mutations in promoter and exons 2-4 and 9-11 in patient with gastric cancer by PCR-SSCP in Chaharmahal Va Bakhtiari province. IRANIAN SOUTH MEDICAL JOURNAL, 14 (4).

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Abstract

Background: Gastric cancer is one of the most important diseases and after lung cancer, is the second cause of cancer death worldwide. Genetic factors including oncogenes and tumor suppressor genes are always contributed in progression of this cancer. The P53 tumor suppressor gene has a broad role in genomic stability and DNA repair. The aim of this study was to determine the P53 gene mutations in gastric cancer specimens in Chaharmahal Va Bakhtiari Province. Methods: In this descriptive-lab based study, we investigated the promoter, exons 2-4 and 9-11 of P53 gene mutations in 38 paraffin embedded gastric cancer specimens. DNA was extracted following the standard phenol chloroform protocol. The P53 gene mutations were determined using PCR-SSCP procedure. Results: Our study revealed no P53 gene mutation in promoter and exons 2-4 and 9-11 in the gastric cancer subjects studied. Conclusion: While P53 gene mutations have been reported as the most frequent genetic alterations and are found in about 50% of the human malignancies, no mutation was detected in this study. The reason may be due to small sample size or mutations on other genes or epigenetic factors.

Item Type: Article
Uncontrolled Keywords: gastric cancer, mutation, P53 gene, promoter, PCR- SSCP
Subjects: WI Digestive System
QZ pathology-Neoplasms
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine > Department of Clinical Sciences > Department of Internal Medicine
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 21 Nov 2017 04:50
Last Modified: 31 Jan 2018 05:50
URI: http://eprints.skums.ac.ir/id/eprint/6374

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