The Effect of miR-372 on Genome Instability in MKN-45 Cell Line

Ghasemi, Soraya. and Mozdarani, Hossein. and Soleimani, Masoud. (2015) The Effect of miR-372 on Genome Instability in MKN-45 Cell Line. Journal of Isfahan Medical School, 32 (311).

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Abstract

Background: Gastric cancer is one of the most common cancers in the world and the second leading cause of cancer mortality in humans. MicroRNAs are a group of endogenous RNA, small non-coding nucleotides in length of 21-23. Overexpression of miR-372 acts as an oncomir in various types of cancer via down-regulation of its target, LATS2. Down-regulation of LATS2 leads to the loss of cell cycle regulation, apoptosis inhibition, and increased proliferation rate of the cells. Methods: In this study, we increased the expression of miR-372 with lentivirus transduction inside the GC cell line MKN-45. After selection of positive cells, miR-372 and LATS2 expression levels were measured through real-time polymerase chain reaction (RT-PCR) assay. Cytochalasin B blocked (MN) assay was done to verify the presence or absence of MN for comparing genomic instability in treated cells compared to the controls. Findings: In the treated cells, compared with the controls, the amount of miR-372 expression significantly increased. Fold changes in 7, 14 and 21 days after the transduction were 7.85, 50.22 and 114.68, respectively (P = 0.030). In contrast to the control cells, the fold changes of LATS2 expression in these days were 0.39, 0.29 and 0.15, respectively (P = 0. 016). In addition, compared with control cells, the genomic instability of treated cells increased significantly (P < 0.001). Conclusion: These results indicate that in MKN-45 cell line, LATS2 is a target of miR-372. LATS2 is down-regulated with increased expression of miR-372. Reduce LATS2, leads to genomic instability during cell division and creates micronuclei and hence may be an important tumor suppressor.

Item Type: Article
Uncontrolled Keywords: Gastric cancer (GC), miR-372, LATS2, Genomic instability
Subjects: WI Digestive System
QZ pathology-Neoplasms
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: Users 1 not found.
Date Deposited: 07 Nov 2017 05:51
Last Modified: 28 May 2018 05:44
URI: http://eprints.skums.ac.ir/id/eprint/6274

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