Comparison of the performance of Disk diffusion, Agar screening and E-test methods with Real- time PCR for the detection of methicillin resistant coagulase negative Staphylococcus strains isolated from clinical samples of Shahrekord university hospitals, 2008

Shariati, Laleh. and Validi, Majid. and Shojapour, Mana. and Hasheminia, Alimohammad. and Tabatabaiefar, Mohammad Amin. and Karimi, Ali. and Nafisi, Mohammad Reza. (2012) Comparison of the performance of Disk diffusion, Agar screening and E-test methods with Real- time PCR for the detection of methicillin resistant coagulase negative Staphylococcus strains isolated from clinical samples of Shahrekord university hospitals, 2008. Iranian South Med J, 15 (2).

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Abstract

Background : Coagulase Negative staphylococci (CoNS) are nosocomial pathogens. The main objective of the study was to compare the performance of disk diffusion, E-test and agar screening methods with Real-time PCR technique for detection of methicillin resistance in coagulase negative staphylococci (MRCoNS), using Taqman® Real-time PCR for mecA DV WKH ³ JROG VWDQ GDUG´ FRP SDULVRQ DVVD\ � Methods : 88 coagulase negative Staphylococcus isolates were identified out of 284 Staphylococcus isolates collected from Hajar and Kashani hospitals-shahrekod, Iran. Methicillin resistance strains were identified by several methods: Disk diffusion, Agar screening, E-test and Real-time PCR. The results of the tested methods ZHUH FRP SDUHG ZLWK WKRVH RI WKH 5 HDO� WLP H 3& 5 E\ & KL VTX DUH RU ) LVKHU¶ V H[ DFW WHVWV� Results : Of the 88 coagulase negative Staphylococcus isolates tested, 46 isolates (52.3%) were mecA - positive and 42 isolates (47.7%) were mecA-negative. The results of all the tested methods had a statistically significant agreement with those of Real-time PCR. The E-test was 100% sensitive and specific for mecA presence. The sensitivity and specificity of oxacillin agar screen (6.0 µg/ml) method were 96% and 98%, respectively and the sensitivity and specificity of oxacillin Disk diffusion method were 91% and 90%, respectively. Conclusion: In the present study, E-test is proposed as the best phenotypic method. In case economic issue matters, the oxacillin agar screening method (6.0 µg/ml), which is suitable for the detection of MRCoNS due to its accuracy and low cost, is recommended. Keywords : methicilli

Item Type: Article
Uncontrolled Keywords: methicillin resistant coagulase negative staphylococcus, disk diffusion, agar screen, Etest, mecA gene, real- time PCR
Subjects: WC Communicable Diseases > Infection.Bacterial Infections
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: zahra bagheri .
Date Deposited: 25 Oct 2017 10:16
Last Modified: 08 May 2018 04:29
URI: http://eprints.skums.ac.ir/id/eprint/6100

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