The Effect of miR-372 on Cell Proliferation in MKN-45 cell line

Ghasemi, Soraya. and Mozdarani, Hossein. and Soleimani, Masoud. (2014) The Effect of miR-372 on Cell Proliferation in MKN-45 cell line. Bull. Env. Pharmacol. Life Sci, 3.

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Abstract

Gastric cancer (GC) is one of the most common cancers and the world's second leading cause of cancer death in human. MiR-372 acts as an oncomir and may disturb cell cycle, apoptosis, invasion, and cell proliferation in various types of cancer by its target LATS2. In this study, we increased the expression of miR-372 through transduction with lentivirus inside the GC cell line MKN-45. After selection of positive cells, total RNA was extracted and Quantitative Real-Time PCR (QRT-PCR) was performed for miR-372 and LATS2. MTT assay was used for evaluating of cell proliferation in treated and control cells. The amount of miR-372 expression significantly increased compared to the control cells (fold changes in 7, 14 and 21 days after transduction; 7.85, 50.22 and 114.68; P=0. 03). In contrast to the control cells the level of LATS2 expression was reduced (fold changes in 7, 14 and 21 days after transduction; 0.39, 0.29 and 0.15; P=0. 016). In addition, the proliferation of treated cells increased significantly (P<0.05). These results indicate that LATS2 is a target of miR-372 and it may be an important tumor suppressor in GC MKN-45 cell line.

Item Type: Article
Uncontrolled Keywords: Key words:Gastric cancer (GC), MKN-45, miR-372, LATS2
Subjects: QU Biochemistry > Cell biology and genetics
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Depositing User: Users 1 not found.
Date Deposited: 17 Oct 2017 08:03
Last Modified: 28 May 2018 05:48
URI: http://eprints.skums.ac.ir/id/eprint/6010

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