Molecular characterization of the human and sheep hydatid cyst strains in Chaharmahal va Bakhtiari province of Iran using restriction fragment length plolymorphism (PCR RFLP)

Moazeni-Bistgani, Mohammad. and Taghipoor, Simin. and Abolhasani, Marziyeh. and Hashemzadeh-Chaleshtori, Morteza. and Zarean, Elham. and Yousofi Darani, Hossein. (2013) Molecular characterization of the human and sheep hydatid cyst strains in Chaharmahal va Bakhtiari province of Iran using restriction fragment length plolymorphism (PCR RFLP). Cell biology, 2 (2).

[img]
Preview
Text
8.pdf

Download (824kB) | Preview

Abstract

Background: Hydatidosis caused by larval stage of Echinococcus granulosus is a cosmopolitan zoonotic infection. In endemic area for this disease there is considerable genetic variation among different isolates of the parasite. These variations may affect the epidemiology, pathology and control of the disease. In this work strain identification of hydatid cysts isolated from human or sheep in Chaharmahal va Bakhtiari province of Iran has been investigated. Method: Fertile sheep hydatid cysts were collected from several abattoirs inChaharmahal vaBakhtiari province ofIran. Human isolates were obtained at surgery from Kashani hospital in the same area. DNA was extracted from preserved protoscoleces and Nested PCR was performed on the extracted DNA samples, the rDNA-ITS fragment was amplified subsequently. Using 4 restriction enzymes include Rsa², HpaII, Alu² and taq², PCR-RFLP procedure was performed on the PCR products. Results: The size of PCR product in this research was 1000bp in both human and sheep isolates. UsingAlu² enzyme; three fragments of 100, 180 and 720bp in human isolates and two fragments of 800bp and 200bp in sheep isolates were created. Rsa² also revealed three segments of 150, 180 and 670bp in human samples and two fragments of 655bp and 345bp in sheep samples. After using HpaII enzyme three segments with 120, 200 and 680bp length in human isolates and two fragments with 700bp and 300bp in sheep isolates were detected. Finally using Taq² enzyme no digestion was occurred on human or sheep samples. Conclusion: The result of this investigation showed that human hydatid cyst strain in Chaharmahal & Bakhtiati province of Iran is different from sheep ones, so it is recommended to recognize DNA sequence in this human samples in future studies.

Item Type: Article
Uncontrolled Keywords: Hydatid cyst; RFLP; Strain; Human; Sheep.
Subjects: WC Communicable Diseases > Tropical and parasitic Diseases
QU Biochemistry > Cell biology and genetics
Divisions: Faculty of Medicine
Depositing User: Users 1 not found.
Date Deposited: 15 Oct 2017 06:24
Last Modified: 19 May 2018 04:00
URI: http://eprints.skums.ac.ir/id/eprint/5899

Actions (login required)

View Item View Item